依达拉奉对创伤性脑损伤大鼠神经细胞凋亡影响.docVIP

依达拉奉对创伤性脑损伤大鼠神经细胞凋亡影响【摘 要】目的研究依达拉奉对创伤性脑损伤大鼠脑组织细胞凋亡及Bcl-2蛋白表达的影响。方法 72只SD 大鼠随机分为依达拉奉治疗组、对照组和假手术组，按伤后6、12、24、48 h分成12组（每组6只）。建立大鼠自由落体脑损伤模型（Feeney′s model），用免疫组织化学染色及原位细胞凋亡检测法（TUNEL法）测定各个组大鼠伤后6、12、24、48 h脑组织Bcl-2蛋白表达和凋亡细胞数。结果 依达拉奉治疗组各时间点细胞凋亡数均明显小于对照组及假手术组的（P＜0．01）；各个时间点的Bcl-2蛋白表达均明显高于对照组及假手术组的（P＜0．01）。结论 依达拉奉可以上调创伤性脑损伤大鼠脑组织的Bcl-2蛋白表达，抑制细胞凋亡。 【关键词】 依达拉奉；创伤性脑损伤；细胞凋亡;Bcl-2 Effect of edaravone on cell apoptosis and expression of bcl-2 following traumatic brain injury in ratsWANG Feng-he．Department of Neurosurgery，People′s Hospital of Yanzhou，Shandong 272000,China 【Abstract】 Objective To investigate the effect of edaravone on the cell apoptosis and the expression of bcl-2 following traumatic brain injury in rats．Methods 72 SD rats were randomly divided into 3 groups：edaravone -treatedgroup,controlgroup andsham group，and were further divided into 6，12，24，48 hours subgroups according to the time of injury(n=6)．Based upon the Feeney′s model，immunohistochemical method and TUNEL staining were used to determine the expression of the bcl-2 protein and number of TUNEL-positive apoptotic cells in the brain tissue at6，12，24，48 hours after injury．Results After 6，12，24，48 hours of injury,the number of TUNEL-positive apoptotic cells in the edaravone-treated group were obviously smaller than that in the control group and sham group (P＜0．01),while，the expression of bcl-2 protein was obviously higher in the edaravone-treated group than that in the control group and sham group(P＜0．01)．Conclusion Edaravone inhibits the neuronal apoptosis following traumatic brain injury in rats,which might be relation with the up-regulated bcl-2 protein expression． 【Key words】 Edaravone；Traumatic brain injury；Cell apoptosis；Bcl-2 创伤性脑损伤(traumatic brain injury,TBI) 在临床常见,病死率高,后遗症多,对患者危害较大。目前认为脑损伤后迟发性神经元凋亡是产生继发性脑损害的主要途径[1]，如何阻止脑损伤后迟发性神经元凋亡是当前临床治疗脑损伤的研究热点之一。目前，依达拉奉对创伤性脑损伤后大鼠神经细胞凋亡影响的研究少见报道，现报告如下。 1 材料与方法 1．1 主要实验仪器与试剂 自制改进型Feeney 自由落体脑损伤装置。原位细胞凋亡检测试剂盒，购自Roche公司。Bcl-2 及Bax免疫组化染色试剂盒，购自武汉博士德生物