Cell Line Development WuXi AppTec（开发无锡AppTec细胞株）.pdf

Cell Line Development Producing high-expressing, stable cell banks from DNA ILLUSTRATION: CHO cell at 10,500x Cell Line Development Proven, high-yielding development platforms accepted by regulatory agencies worldwide WuXi AppTec, a global RD and GMP/GLP contract manufacturing and testing company, oers comprehensive cell line development capabilities that start at DNA sequences provided by clients and nish with the delivery of high-yielding stable single clones. We work with you to stay within your timeline and to select top clones according to their productivity and quality. The WuXi AppTec Program Construction Selection of Fed-batch Long-term of high- Transfection initial stable Cloning evaluation productivity Final expression producing top clones genetic reports vectors pools selection stability study Construction of high-expression vectors WuXi AppTec oers gene-specic cloning into commercial vectors (e.g., Invitrogen), or client-specied vector systems. The integrity of the target gene cloned into the vector will be conrmed by sequencing prior to transfection into a host cell line. Selection of initial stable producing pools Transfected cells are grown in selective media to generate stable