当归体外对山梨醇生成及蛋白质糖基化抑制作用.docVIP

当归体外对山梨醇生成及蛋白质糖基化抑制作用摘要：目的观察当归水提取液对红细胞内山梨醇生成及糖化蛋白 （BSA）生成的影响。方法当归水提取液与红细胞、葡萄糖混合，培养2 h，测定山梨醇的含量，计算当归提取液对红细胞山梨醇生成的抑制率。不同浓度的当归提取取液加入体外蛋白糖化终末产物生成系统，培养8周，测定孵育液的荧光值，计算当归对糖化蛋白生成的抑制率。结果当归水提取液使高浓度葡萄糖培养的红细胞内山梨醇生成减少，糖化蛋白生成较对照组少。结论当归水提取液能抑制红细胞内山梨醇的生成，抑制蛋白质的糖基化作用。 关键词：当归；山梨醇；蛋白质糖基化 中图分类号：R282.71文献标识码：A文章编号：1672-979X(2007)06-0015-02 Inhibitory Effect of Angelica Extract on Content of Sorbitol in Red Blood Cell and Protein Glycosylation in Vitro GONG Jian-wei1, TIAN En-sheng 2 (1. Shandong Zibo Prevention and Treatment Center for Occupational Diseases, Zibo 255067, China; 2. Shandong Zibo Institute for Drug Control, Zibo 255067, China) Abstract：Objective To investigate the effect of angelica extract on the content of sorbitol in red blood cell and the synthesis of glycated protein in vitro. Methods Glucose, red blood cell and the angelica extract were mixed. The mixture was cultivated for two hours. The inhibition rate was calculated according to the concentration of sorbitol. The angelica extract was added to the system of glycosylated protein. The system was cultivated for eight weeks. The inhibition rate was calculated according to fluorescence value of the system. Results The angelica extract decreased the production of sorbitol in red blood cell and also decreased the production of glycated protein in the incubated liquid. Conclusion The angelica extract can inhibit the production of sorbitol and protein glycosylation. Key words：angelica; sorbitol; protein glycosylation 糖尿病（diabetes methitus, DM）是常见的以高血糖和代谢紊乱为主要特征的内分泌代谢疾病。据统计，目前全世界约有1.2亿糖尿病患者，且患病率仍在快速增加。长期高血糖状态危害体内许多组织和器官，导致多种糖尿病慢性并发症（diabetic chronic complications, DCC），如神经病变、视网膜病变、肾病等。目前提出的病因学包括改变的多元醇代谢、超氧自由基的产生、蛋白糖化及血管供血不足等。当归是一种常用中药，在《神农本草经》中列为中品，具有补血活血、祛风湿等功效，在《本草纲目》中列为草部芳香类56种药物之首，临床用途广泛，俗有“十方九归”之说，还被誉为“血家圣药”。现代药理研究表明，当归具有保护肾脏[1]、抑制血小板凝集[2]、调节肝药酶活性[3]及增强免疫力等作用。当归在体外对抗红细胞内山梨醇生成及抑制蛋白质糖基化作用尚未见文献报道。 1材料与方法 1.1材料 当归提取物（淄博市职业病防治院药剂科提供）；氨基胍（Sigma公司）；葡萄糖（沈阳化学试剂厂）；变色酸（上海三爱思试剂有限公司）；山梨醇（北京奥博星生物技