河北不同产地知母药材中芒果苷及菝葜皂苷元含量测定.docVIP

河北不同产地知母药材中芒果苷及菝葜皂苷元含量测定摘 要：目的：考察河北不同产地知母药材中芒果苷及菝葜皂苷元的含量。 方法：分别采用HPLC法和HPLC－ELSD法测定芒果苷和菝葜皂苷元的含量。测定芒果苷以水∶乙腈∶0.85%磷酸（100∶25∶5）为流动相；流速为0.6mL/min；柱温30℃；检测波长为258nm。测定菝葜皂苷元以甲醇∶水（95∶5）为流动相；流速为1.0mL/min；柱温30℃；用蒸发光散色检测器检测，漂移管温度85℃。 结果：河北不同产地知母药材中菝葜皂苷元含量为1.51%～1.73%，芒果苷含量为0.11%～0.73%。 结论：本法测定知母药材中菝葜皂苷元及芒果苷含量，方法简单、快速、准确、重现性好，方法学考察符合含量测定的要求，可以用来控制知母药材质量。? 关键词：知母；芒果苷；菝葜皂苷元；高效液相? 中图分类号：R284.2 文献标识码：A文章编号： 1673-7717（2008）11-2368-03 ? Dertermination Content of Mangiferin and Sarsasapogenine in ?Rhizoma Anemarrhenae from Different Areas ? HOU Xiu?qing?1，MAO Chun?qin?2，LU Tu?lin?2，SONG Shen?2? （1.China Pharmaceuticul University，Nanjing210009,Jiangsu,China；? 2.Nanjing University of Chinese Medicine,Nanjing,210046,Jiangsu,China）? Abstract：Objective:To establish the determination of mangiferin and sarsasapogenine in Rhizoma Anemarrhenae from different areas.Methods:Determination of mangiferin and sarsasapogenine in Rhizoma Anemarrhenae were by HPLC and HPLC－ELSD. The mobile phase were water-acetonitrile-0.85%phosphoric acid（100∶25∶5）and methanol-water（95∶5）,with the flow rate were 0.6mL/min and 1.0mL/min, at 30℃.The detection wavelength of mangiferin was at 258nm. An evaporative light- scattering detector ( ELSD) was used as detector to determine sarsasapogenine with the drift tube temperature of 85℃. Result:The content of mangiferin and sarsasapogenine in Rhizoma Anemarrhenae from different areas were 0.11%～0.73% and 1.51%～1.73%. Conclusion:The method can be used for the equality control of Rhizoma Anemarrhenae from different areas,the result have provide a scientific basis for processing Rhizoma Anemarrhenae. Key words：Rhizoma Anemarrhenae; mangiferin; sarsasapogenine；HPLC ? 知母是百合科（liliaceae）植物知母(Anema rrhena asphodeloidesBge)的干燥根茎，具有清热泻火、生津润燥的功效，用于外感热病、高热烦渴、肺热燥咳、骨蒸潮热、内热消渴、肠燥便秘[1]。知母有效成分为多种甾体皂苷。其皂苷元主要为菝葜皂苷元(sarsasapogenin)。因菝葜皂苷元为甾体皂苷元，分子中没有共扼结构，无紫外特征吸收，对样品的检测造成了一定的难度[2]。为此，本实验采用高效液相-蒸发光散射检测器( ELSD)测定知母中菝葜皂苷元的含量。芒果苷是知母中清热作用的有效成分。同时具有免疫活性、抗菌活性、