长期饲喂高精料日粮导致的SARA效应对泌乳期山羊乳品质及肝脏中基因表达的影响.pdfVIP

科技创新导报 2016 NO.07 Science and Technology Innovation Herald 科技报告导读 代谢物，LPS-L和LPS-H之间的差异代谢物有3种，这些代谢物主要与蛋白质、脂肪和碳水化合物等代谢途径相关，可作为LPS 肝损伤状态下的标志物。1H-NMR可以全面地检测到肝脏在LPS攻击下代谢状态的变化，准确地筛选到LPS致奶山羊肝损伤的标 志代谢产物，为判断LPS影响肝脏相关代谢的途径提供信息，为科学全面地阐明LPS影响肝脏的机理提供理论依据。 关键词：代谢组学 脂多糖 肝脏 代谢物 奶山羊 Study of the Effect of Lipopolysaccharide on Hepatic Metabolism in Dairy Goat Liver Based on 1H-NMR and Morphology Wang Linfeng Jia Shaodan Yang Gaiqing Zhu Heshui Yang Guoyu (Henan Agricultural University) Abstract In order to research the changes of dairy goat liver metabolites by using 1H-NMR test via molecular markers and explore the ： influence of lipopolysaccharide (LPS) on dairy goat liver.Total 15 Guanzhong dairy goats of 12-month-old with 24～28 kg live body weight (BW) were selected and divided into three groups randomly, i.e. control group, LPS-L group and LPS-H group. Animals could access to total mixed ration feeds and water ad libitum. At beginning of the experiment (0 h), the goats in LPS-L and LPS-H groups were given 20 and 40 μg/kg BW of LPS solution via intraperitoneal injections, respectively. The goats in CTL were received same volume of saline water. 24 h later, goats in each LPS treated groups were added LPS solution once again.At the time point of 0 h, 24 h and 48 h, blood sample were drawn from neck venous and were used to extract serum after centrifuging for biochemical determination. 48 h later, the goats’ liver tissue samples were collected by biopsy and stored in liquid nitrogen. Metabolites in liver tissue were detected by 1H- NMR and analyzed by partial least squares discriminant analysis (PLS-DA) combined with t-test to identify metabolic differences under