饥饿应激对藏系绵羊肌肉AMPK活性、糖酵解及品质的影响.pdfVIP

畜牧与兽医 2014 年第46 卷第9 期 饥饿应激对藏系绵羊肌肉 AMPK 活性、 糖酵解及品质的影响 商振达，毛学荣\李长忠，余忠祥，雷良煌，阎明毅，孔庆辉 (青海大学，青海西宁 81∞16) 摘要:通过分别测定不同饥饿时间后藏系绵羊肌肉的AMPK 活性，糖酵解指标以及肌肉品质指标，分析饥饿应激对宰后 AMPK 活性及藏系绵羊 肉品品质的影响，初步探明饥饿应激与 AMPK 活性及肌肉品质的关系，为提高肉品品质提供理论基础。结果表明.短期的饥饿应激(不超过24 h) 对宰后藏系绵羊肌肉的 AMPK 活性以及肌肉的品质没有显著的影响，而长期的饥饿应激(超过48 h) 可以使宰后藏系绵羊肌肉的 AMPK 活性 升高， ACC 和 GS 活性下降，糖原含量下降，乳酸含量升高， pH 值下降，熟肉率和滴水损失下降，剪切力上升。结果提示长时间的饥饿应激 (超过48 h) 可以通过提高 AMPK 活性，加快糖酵解的速度，使宰后藏系绵羊肌肉的糖原含量下降，乳酸含量升高， pH 值降低，而较低的 pH 值，对藏系绵羊的肌肉品质有很大的影响。 关键词:藏系绵羊;饥饿应激; AMPK 活性;糖酵解:肉晶晶质 中图分类号: S826.8 文献标志码:A 文章编号: 0529-5130( 2014 )09-∞01-05 Effects of hunger stress on AMPK activity , glycolysis and muscle quality in Tibetan sheep SHANG Zhen巾， MAO Xueroni ，口 Changzhong ， YU Zhongxia吨， LEI Liangyu , YAN Mingyi , KONG Qingh山 (Qinghai University , Xining810016 , China) Abstract: To analysis hunger stress on AMPK activity and Tibetan sheep muscle quality after slaughter , the AMPK activity , glyco1ysis indi- cators and muscle quality indicators in Tibetan sheep after different starvation time were measured for theoretical foundation to improve meat quality. The resu1ts showed that short-term hunger stress had no significant influence to AMPK activity and muscle qua1ity in Tibetan sheep af- ter slaughter , but 10ng-term hunger stress to Tibetan sheep cou1d make increases of the AMPK activity , decreases of the ACC and GS activity , decreases of glycogen contents , increases of LD contents , decreases of the pH va1ue , decreases of cooked meat rate and drip 10ss , increases of the shearing force. lρng-term hunger stress cou1d make the decrease of glycogen 1evels and increases of 1actic acid contents , and the 10wer pH of Tibetan sheep