betaine treatment attenuates chronic ethanol-induced hepatic steatosis and alterations to the mitochondrial respiratory chain proteome甜菜碱治疗慢性ethanol-induced肝脂肪变性变弱和改变线粒体呼吸链蛋白质组.pdfVIP

Hindawi Publishing Corporation International Journal of Hepatology Volume 2012, Article ID 962183, 10 pages doi:10.1155/2012/962183 Research Article Betaine Treatment Attenuates Chronic Ethanol-Induced Hepatic Steatosis and Alterations to the Mitochondrial Respiratory Chain Proteome Kusum K. Kharbanda,1, 2 Sandra L. Todero,1 Adrienne L. King,3 Natalia A. Osna,2 Benita L. McVicker,2 Dean J. Tuma,2 James L. Wisecarver,4 and Shannon M. Bailey3 1 Research Service-151, Veterans Aff airs Nebraska-Western Iowa Health Care System, 4101 Woolworth Avenue, Omaha, NE 68105, USA 2 Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68198, USA 3 Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA 4 Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA Correspondence should be addressed to Kusum K. Kharbanda, kkharbanda@ Received 29 July 2011; Accepted 31 August 2011 Academic Editor: Laura Schrum Copyright © 2012 Kusum K. Kharbanda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. Mitochondrial damage and disruption in oxidative phosphorylation contributes to the pathogenesis of alcoholic liver injury. Herein, we tested the hypothesis that the hepatoprotective actions of betaine against alcoholic liver injury occur at the level of the mitochondrial proteome. Methods. Male Wister rats were pair-fed control or ethanol-containing liquid diets supplemented with or without betaine (10 mg/mL) for 4-5 wks. Liver was examined for triglyceride accumulation, levels o