biological effects of osteoblast-like cells on nanohydroxyapatite particles at a low concentration range生物效应的osteoblast-like细胞nanohydroxyapatite颗粒在低浓度范围.pdfVIP

Hindawi Publishing Corporation Journal of Nanomaterials Volume 2011, Article ID 104747, 6 pages doi:10.1155/2011/104747 Research Article Biological Effects of Osteoblast-Like Cells on Nanohydroxyapatite Particles at a Low Concentration Range Xiaochen Liu,1 Jie Wei,1, 2 and Shicheng Wei1, 3 1 Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China 2 Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China 3 Department of Oral and Maxillofacial Surgery, School of Stomatology, Peking University, Beijing 100081, China Correspondence should be addressed to Jie Wei, nic750@263.net and Shicheng Wei, sc-wei@ Received 29 April 2011; Revised 19 July 2011; Accepted 19 July 2011 Academic Editor: Somchai Thongtem Copyright © 2011 Xiaochen Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The biological effects of osteoblast-like MG-63 cells on nanohydroxyapatite (n-HA) at the low concentration range (5–25 μg/mL) for 5 days was investigated. The results showed the viability and actin cytoskeleton of the cells descended with the increase of the concentration of n-HA, and the actin cytoskeleton of cells was depolymerised and became more disordered. Apoptotic rate of cells (1.85%, 1.99%, and 2.29%) increased with the increase of n-HA concentration (5, 15, and 25μg/mL) and become significantly higher than the control. Total intracellular protein content decreased with n-HA concentration increase, showing significant difference