selective gene transfer to the retina using intravitreal ultrasound irradiation选择性基因转移使用intravitreal超声波照射视网膜.pdfVIP

Hindawi Publishing Corporation Journal of Ophthalmology Volume 2012, Article ID 412752, 5 pages doi:10.1155/2012/412752 Research Article Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation Shozo Sonoda,1 Katsuro Tachibana,2 Toshifumi Yamashita,1 Makoto Shirasawa,1 Hiroto Terasaki,1 Eisuke Uchino,1 Ryo Suzuki,3 Kazuo Maruyama,3 and Taiji Sakamoto1 1 Department of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan 2 Department of Anatomy, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan 3 Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Sagamihara 229-0195, Japan Correspondence should be addressed to Shozo Sonoda, shou@m3.kufm.kagoshima-u.ac.jp Received 14 September 2011; Revised 30 October 2011; Accepted 30 October 2011 Academic Editor: Edward Manche Copyright © 2012 Shozo Sonoda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vitrectomy system utilizing a small probe. After vitrectomy, the US probe was inserted through a scleral incision. A mixture of GFP plasmid (50 µL) and bubble liposomes (BLs; 50 µL) was injected into the vitreous cavity, and US was generated to the retina using a SonoPore 4000. The control group was not exposed to US. After 72 h, the gene-transfer effici