ITS1序列分析在凤鲚中的应用研究(修9P).docVIP

凤鲚ITS1和Cyt b基因序列的比较研究( 孙 超刘洪波姜涛杨 健南京农业大学无锡渔业学院 无锡 214081；中国水产科学研究院淡水渔业研究中心，中国水产科学研究院内陆渔业生态环境与资源重点开放实验室 无锡 214081 comparative study between ITS1 sequence and Cyt b gene sequence in Coilia mystus SUN Chao①, LIU Hong-bo②, JIANG Tao①,YANG Jian①②* ① Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; ② Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China Abstract: Coilia mystus is a very important economic fish in Yangtze River estuary. Through comparison with Cyt b gene sequence, this study presented the first investigation to reveal ITS1 sequence characteristics and its potential utilization for genetic diversity and kinship measurements for this species and other Coilia species. The results indicated that the GC content of ITS1 sequence (71.19%) was higher than Cyt b gene sequence (42.96%). Also, the haplotype and nucleotide diversity of ITS1 sequence (0.900/0.00997±0.01116) was higher than that of Cyt b gene sequence (0.400/0.000369±0.000469). Both MCL and K2P genetic distances of ITS1 were at interspecies level between C. mystus and C. nasus, which were coincident with those of Cyt b gene sequences. The ML and UPGMA trees constructed with ITS1 sequences and Cyt b gene sequences suggested that C. mystus and C. nasus formed the separate monophyletic groups, respectively. Hence, the consistency of analyses results between ITS1 sequence and Cyt b gene sequence were good, so the ITS1 sequence could be used as a good genetic marker for genetic diversity and kinship measurements for C. mystus and other Coilia species (e.g. C. nasus). Keyword: ITS1; genetic diversity; C. mystus; kinship analysis 凤鲚（Coilia mystus）属鲱形目Clupeiforms），鳀科Engraulidae），鲚属Coilia），为长江口的一种重要的经济鱼类，但由于环境污染和过度捕捞等因素的危害，目前资源量已显著下降[1]。为了有效地保护其物种多样性特征和种质资源，同时开发一些重要的资源管理的技术手段（如鱼卵、仔幼鱼的鉴定等），有必要利用重复性好，灵敏度高的分子生物学方法[2]，开展凤鲚遗传多样性及与其它鲚属鱼类间的亲缘关系等方面的基础研究。 分子标记的选择对于研究物种内的遗传多样性