胎猪脱细胞主动脉作为小口径组织工程血管替代物体内试验探究.docVIP

胎猪脱细胞主动脉作为小口径组织工程血管替代物体内试验探究[摘要]目的：研究胎猪脱细胞主动脉（decellularized aorta of fetal pigs，DAFP）的生物相容性，确定其是否有作为支架材料用于小口径组织工程血管移植的潜力。方法：利用胰酶和核酸酶联合的脱细胞方法来制备胎猪主动脉脱细胞基质(DAFP)，将其作为小口径组织工程血管的生物支架材料移植在成年犬单侧颈总动脉处，并监测其移植处的血流通畅情况，后期又通过组织学染色观察组织工程血管的组织学结构；扫描电镜观察血管的内表面结构；透射电镜观察其内表面的内皮细胞再生情况。结果：组织学染色结果表明构建的小口径组织工程血管具有完整的内膜层及中膜层结构；扫描电镜结果显示组织工程血管内表面覆盖着完整的内皮细胞层。结论：DAFP有作为小直径组织工程血管支架用于体内移植的潜力。 [关键词]组织工程；血管移植；生物支架材料；细胞外基质 [中图分类号]R318 [文献标识码]A [文章编号]1008-6455（2012）05-0773-03 The vivo study on decellularized aorta of fetal figs can be substituted for tissue engineered small-diameter blood vessel ZHANG Ying-bo,LIU Guo-feng,LI Qing-chun,YANG Da-ping (Department of Plastic Surgery,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,Heilongjiang,China) Abstract: Objective To study the biocompatibility of the DAFP,determining it weather have a potential as a scaffold of tissue engineered small-diameter blood vessel graft. Methods Carotid artery of fetal pig was removed cells by using Trypsin／EDTA,ribonuclease and desoxyribonuclease.Afterward， we transplanted the DAFP into unilateral carotid artery of 6 adult dogs individually.Both the DAFP and the tissue which have been transplanted were evaluated with Hematoxylin and Eosin (HE) staining,scanning electron microscopy and transmission electron microscopy. Results H＆E staining and electron microscopy confirmed that carotid arteries have intact monolayer endothelial cells. Conclusion Decellularized aorta of fetal figs have the ability to substitute for further small-diameter vascular tissue engineering. Key words:tissue engineering， vascular graft， biological scaffold， extracellular matrix 动脉粥样硬化及周围血管疾病是世界范围内危害人类健康的主要疾病， 并且发病率逐年上升[1]，病情严重的患者常需要通过小口径血管(内径 1.4 术后观测：移植术后观测手术区域有无出血及血肿形成，动物有无发热、呼吸急促等生命体征的变化，并给予及时的处理。术后定期观察实验动物的生存状态，分别于术后10天、1个月，3个月及6个月的时期利用彩色多普勒进行血流通畅度的观测。 1.5 Ⅱ期手术：在确定移植入的血管支架已基本被宿主所接纳（无明显排斥反应）；血流通畅度良好；支架与正常血管的吻合处管壁内表面光滑后(约6个月后)，再次手术取出移植处的血管组织。术前准备同Ⅰ期手术；在原切口附近再次切开，剥离粘连组织，并切取移植入的支架血管和