多维气相气质联用法测定葡萄酒中的氨基甲酸乙酯.pdfVIP

2016 Vol.35 No.4 154- Serial No.290 China Brewing Analysis and Examination 多维气相/气质联用法测定葡萄酒中的氨基甲酸乙醋 刘伟l ，孙谦2 ，范军3 ，邓晓丽3 ，李月琪4，杨桂香4，黄涛宏3 (1.山东省食品药品检验研究院，山东济南 250101;2. 岛津企业管理{中国)有限公司，陕西西安 710065; 3. 岛津企业管理(中国)有限公司，上海 200052; 4. 岛津企业管理(中国)有限公司，北京 100020) 摘要:建立了多维气相/气质联用CMDGC/GCMS) 法测定葡萄酒中氨基甲酸乙酶的检测方法。采用硅藻土固相萃取柱对葡萄酒中氨 基甲酸乙酶CEC) 进行提取净化，利用MDGC/GCMS对葡萄酒中EC进行检测。结果表明，葡萄酒中EC与干扰物均得到很好分离，EC在 0~1 000μgIL范围内线性关系良好CR=0.999 3) ，检出限CS/N=3) 为1.0μgIL，加标回收率为82.97%~86.82%，相对标准偏差CRS因为 2.29%; 方法的精密度和重复性试验RSD分别为2.42%和2.88%。表明该方法准确度高、重复性和精密度良好，适用于葡萄酒中的氨基 甲酸乙酶的检测。 关键询:多维气相/气质联用法:葡萄酒;氨基甲酸乙酶 中图分类号;T8262 文章编号;0254-5071 (2016) 04-0154-04 doi:10.1188剖.issn.0254-5071.2016.04.034 Determination of ethyl carbamate in wine by m u1tidimensiona1 gas chromatography with g臼 chromatography mass spectrometric 3 3 LIU Wei, SUN Qi时， FAN Jun , DENG Xiao Ii , LI Yueqi4, YANG Guixiang飞 HUANG Taohong3 (J .Shandong Institute for F.∞d and Drug Control, Jinan 250/0 1, China; 2.Shimadzu (China) Co., Ltd. ,Xian 7/0065, China; 3.Shimadzu (China) Co. , Ltd. , Shan.βai2，∞052， China; 4.S挝madzu (China) Co., Ltd. , Beijing 100020, China) Ab由act: The determination method of ethyl carbamate (EC) in wine was established by multidimensional gas chromaωgraphywi也gas chromatogra- phy mass spectrometric 仙IDGC/GCMS) 刀le EC in wine was extracted and purified by diatomaceous ea由solid phase extraction column, then was detected by MDGC/GCMS. The results showed that the EC and interfering substance in wine were well separated. EC had a g∞d linear relationship in the rage of 0-1 000μgIL (R=0.999 3), the limit of detection (S/N=3) was 1.0μgIL， the adding standard recover rate was 82.97%-86.82%, relative standard d