bone marrow mesenchymal stem cells for improving hematopoietic function an in vitro and in vivo model. part 2 effect on bone marrow microenvironment骨髓间充质干细胞改善造血功能的体外和体内模型。.pdfVIP

Bone Marrow Mesenchymal Stem Cells for Improving Hematopoietic Function: An In Vitro and In Vivo Model. Part 2: Effect on Bone Marrow Microenvironment Soraya Carrancio1,2,3, Belen Blanco1,2,3, Carlos Romo1,2,3, Sandra Muntion1,2, Natalia Lopez-Holgado1,2, 4 ˜ 5 1,2,3 1,2 Juan F. Blanco , Jesus G. Brinon , Jesus F. San Miguel , Fermin M. Sanchez-Guijo , M. Consuelo del ˜ 1,2,3* Canizo ´ ´ 1 Servicio de Hematologıa, Hospital Universitario de Salamanca, Salamanca, Spain, 2 Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y Leon and Red ´ ´ ´ Nacional de Terapia Celular (Tercel, ISCIII), Castilla y Leon, Spain, 3 Centro de Investigacion del Cancer-IBMCC (Universidad de Salamanca-CSIC), Salamanca, Spain, ´ 4 Servicio de Traumatologıa, Hospital Universitario de Salamanca, Salamanca, Spain, 5 Departamento de Biologia Celular y Patologia, Universidad de Salamanca, Spain Abstract The aim of the present study was to determine how mesenchymal stem cells (MSC) could improve bone marrow (BM) stroma function after damage, both in vitro and in vivo. Human MSC from 20 healthy donors were isolated and expanded. Mobilized selected CD34+ progenitor cells were obtained from 20 HSCT donors. For in vitro study, long-term bone marrow cultures (LTBMC) were performed using a etoposide damaged stromal model to test MSC effect in stromal confluence, capability of MSC to lodge in stromal layer as well as some molecules (SDF1