bone marrow-derived mesenchymal stem cells ameliorate hepatic ischemia reperfusion injury in a rat model骨骨髓来源间充质干细胞改善肝缺血再灌注损伤大鼠模型.pdfVIP

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Hepatic Ischemia Reperfusion Injury in a Rat Model 1,2 1 1 1,2 1 Hiroyuki Kanazawa *, Yasuhiro Fujimoto , Takumi Teratani , Junji Iwasaki , Naoya Kasahara , Kouji 1 2 3 1 Negishi , Tatsuaki Tsuruyama , Shinji Uemoto , Eiji Kobayashi 1 Division of Development of Advanced Treatment, Center for Development of Advanced Medical Technology, Jichi Medical University, Yakushiji City, Tochigi, Japan, 2 Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto City, Kyoto, Japan, 3 Department of Hepato-pancreato-biliary Surgery and Transplantation, Graduate School of Medicine, Kyoto University, Kyoto City, Kyoto, Japan Abstract Background: Ischemia-reperfusion (I/R) injury associated with living donor liver transplantation impairs liver graft regeneration. Mesenchymal stem cells (MSCs) are potential cell therapeutic targets for liver disease. In this study, we demonstrate the impact of MSCs against hepatic I/R injury and hepatectomy. Methodology/Principal Findings: We used a new rat model in which major hepatectomy with I/R injury was performed. Male Lewis rats were separated into two groups: an MSC group given MSCs after reperfusion as treatment, and a Control group given phosphate-buffered saline after reperfusion as placebo. The results of liver function tests, pathologic changes in the liver, and the remnant liver regeneration rate were assessed. The fate of transplanted MSCs in the luciferase- expressing rats was examined by in vivo luminescent imaging. The MSC