comparative component analysis of exons with different splicing frequencies比较不同的外显子剪接频率的成分分析.pdfVIP

Comparative Component Analysis of Exons with Different Splicing Frequencies 1,2 2 1 3,4 1 2 Shiqin Song , Qianli Huang , Jiaming Guo , Jesse Li-Ling , Xueping Chen *, Fei Ma * 1 Department of Chemistry, University of Science and Technology of China, Hefei, China, 2 College of Life Science, Nanjing Normal University, Nanjing, China, 3 Department of Medical Genetics, China Medical University, Shenyang, China, 4 Sino-Dutch Biomedical and Information Engineering School, Northeastern University, Shenyang, China Abstract Transcriptional isoforms are not just random combinations of exons. What has caused exons to be differentially spliced and whether exons with different splicing frequencies are subjected to divergent regulation by potential elements or splicing signals? Beyond the conventional classification for alternatively spliced exons (ASEs) and constitutively spliced exons (CSEs), we have classified exons from alternatively spliced human genes and their mouse orthologs (12,314 and 5,464, respectively) into four types based on their splicing frequencies. Analysis has indicated that different groups of exons presented divergent compositional and regulatory properties. Interestingly, with the decrease of splicing frequency, exons tend to have greater lengths, higher GC content, and contain more splicing elements and repetitive elements, which seem to imply that the splicing frequency is influenced by such factors. Comparison of non-alternatively spliced (NAS) mouse genes with alternatively spliced human orthologs also suggested that exons with lower splicing frequencies may be newly evolved ones which gained functions with splicing frequencies altered through the evolution. Our findings