dextran fractional clearance studies in acute dengue infection右旋糖酐部分间隙研究急性感染登革热.pdfVIP

Dextran Fractional Clearance Studies in Acute Dengue Infection 1 1 1 2,4 2 Julie Nguyen-Pouplin *, Thomas Pouplin , Toi Pham Van , Trung Dinh The , Dung Nguyen Thi , Jeremy Farrar1,3, Hien Tran Tinh1,2, Bridget Wills1,3 1 Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam, 2 Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam, 3 Centre for Clinical Vaccinology and Tropical Medicine, Oxford University, Oxford, United Kingdom, 4 University of Medicine and Pharmacy of Ho Chi Minh City, Ho Chi Minh City, Viet Nam Abstract Background: Although increased capillary permeability is the major clinical feature associated with severe dengue infections the mechanisms underlying this phenomenon remain unclear. Dextran clearance methodology has been used to investigate the molecular sieving properties of the microvasculature in clinical situations associated with altered permeability, including during pregnancy and in various renal disorders. In order to better understand the characteristics of the vascular leak associated with dengue we undertook formal dextran clearance studies in Vietnamese dengue patients and healthy volunteers. Methodology/Principal Findings: We carried out serial clearance studies in 15 young adult males with acute dengue and evidence of vascular leakage a) during the phase of maximal leakage and b) one and three months later, as well as in 16 healthy control subjects. Interestingly we found no difference in the clearance profiles of neutral dextran solutions among the dengue patients at any time-point or in comparison to the healthy volunteers. Conclus