drug-eluting fibers for hiv-1 inhibition and contraception为抑制hiv - 1和避孕药物洗脱纤维.pdfVIP

Drug-Eluting Fibers for HIV-1 Inhibition and Contraception Cameron Ball, Emily Krogstad, Thanyanan Chaowanachan, Kim A. Woodrow* Department of Bioengineering, University of Washington, Seattle, Washington, United States of America Abstract Multipurpose prevention technologies (MPTs) that simultaneously prevent sexually transmitted infections (STIs) and unintended pregnancy are a global health priority. Combining chemical and physical barriers offers the greatest potential to design effective MPTs, but integrating both functional modalities into a single device has been challenging. Here we show that drug-eluting fiber meshes designed for topical drug delivery can function as a combination chemical and physical barrier MPT. Using FDA-approved polymers, we fabricated nanofiber meshes with tunable fiber size and controlled degradation kinetics that facilitate simultaneous release of multiple agents against HIV-1, HSV-2, and sperm. We observed that drug-loaded meshes inhibited HIV-1 infection in vitro and physically obstructed sperm penetration. Furthermore, we report on a previously unknown activity of glycerol monolaurate (GML) to potently inhibit sperm motility and viability. The application of drug-eluting nanofibers for HIV-1 prevention and sperm inhibition may serve as an innovative platform technology for drug delivery to the lower female reproductive tract. Citation: Ball C, Krogstad E, Chaowanachan T, Woodrow KA (2012) Drug-Eluting Fibers for HIV-1 Inhibition and Contraception. PLoS ONE 7(11): e49792. doi:10.1371/journal.pone.0049792 Editor: Gilda Tachedjian, Burnet Institute, Australia Received July 27, 2012; Accepted October 12, 2012; Published November 28, 2012 Copyright: 2012 Ball et al. This is an open-access article distributed under the te