e-cadherin acts as a regulator of transcripts associated with a wide range of cellular processes in mouse embryonic stem cells钙粘蛋白作为监管机构与各种相关的记录小鼠胚胎干细胞的细胞过程.pdfVIP

E-Cadherin Acts as a Regulator of Transcripts Associated with a Wide Range of Cellular Processes in Mouse Embryonic Stem Cells 1,2 1 1 1 1 3 Francesca Soncin , Lisa Mohamet , Sarah Ritson , Kate Hawkins , Nicoletta Bobola , Leo Zeef , 2 1 Catherine L. R. Merry , Christopher M. Ward * 1 Core Technology Facility, Faculty of Medical and Human Sciences, The University of Manchester, Manchester, United Kingdom, 2 Materials Science Centre, The University of Manchester, Manchester, United Kingdom, 3 Microarray Facility, Faculty of Life Sciences, Michael Smith Building, The University of Manchester, Manchester, United Kingdom Abstract Background: We have recently shown that expression of the cell adhesion molecule E-cadherin is required for LIF- dependent pluripotency of mouse embryonic stem (ES) cells. Methodology: In this study, we have assessed global transcript expression in E-cadherin null (Ecad-/-) ES cells cultured in either the presence or absence of LIF and compared these to the parental cell line wtD3. Results: We show that LIF has little effect on the transcript profile of Ecad-/- ES cells, with statistically significant transcript alterations observed only for Sp8 and Stat3. Comparison of Ecad-/- and wtD3 ES cells cultured in LIF demonstrated significant alterations in the transcript profile, with effects not only confined to cell adhesion and motility but also affecting, for example, primary metabolic processes, catabolism and genes associated with apoptosis. Ecad-/- ES cells share similar, although not identical, gene expression profiles to epiblast-derived pluripotent stem cells, suggesti