elevated rates of sister chromatid exchange at chromosome ends染色体末端的姐妹染色单体交换率升高.pdfVIP

Elevated Rates of Sister Chromatid Exchange at Chromosome Ends 1 1 1 1 2 1* M. Katharine Rudd , Cynthia Friedman , Sean S. Parghi , Elena V. Linardopoulou , Li Hsu , Barbara J. Trask 1 Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, 2 Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America Chromosome ends are known hotspots of meiotic recombination and double-strand breaks. We monitored mitotic sister chromatid exchange (SCE) in telomeres and subtelomeres and found that 17% of all SCE occurs in the terminal 0.1% of the chromosome. Telomeres and subtelomeres are significantly enriched for SCEs, exhibiting rates of SCE per basepair that are at least 1,600 and 160 times greater, respectively, than elsewhere in the genome. Citation: Rudd MK, Friedman C, Parghi SS, Linardopoulou EV, Hsu L, et al. (2007) Elevated rates of sister chromatid exchange at chromosome ends. PLoS Genet 3(2): e32. doi:10.1371/journal.pgen.0030032 Introduction telomere-repeat probe indicates an SCE in the interval between the signals of the two probes (Figure 1). Chromosome ends participate in frequent recombination For example, Probe Z lies in the most distal part of the events. Human subtelomeric regions have undergone multi- subtelomere just proximal to the telomere-repeat array, so it ple interchromosomal exchanges during meiosis, giving rise monitors SCE events that occur in the ,10-kb interval to the highly duplicated structures pro