simple modification of karl-fischer titration method for determination of water content in colored samples简单的修改费歇尔滴定法测定含水量的彩色样本.pdfVIP

Hindawi Publishing Corporation Journal of Analytical Methods in Chemistry Volume 2012, Article ID 379724, 6 pages doi:10.1155/2012/379724 Research Article Simple Modification of Karl-Fischer Titration Method for Determination of Water Content in Colored Samples ˇ Eva Tavcar, Erika Turk, and Samo Kreft Faculty of Pharmacy, University of Ljubljana, Askerceva cesta 7, 1000 Ljubljana, Slovenia ˇ ˇ ˇ Correspondence should be addressed to Eva Tavcar, eva.tavcar@ffa.uni-lj.si Received 30 November 2011; Accepted 6 March 2012 ´ Academic Editor: Alberto N. Araujo ˇ Copyright © 2012 Eva Tavcar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The most commonly used technique for water content determination is Karl-Fischer titration with electrometric detection, requiring specialized equipment. When appropriate equipment is not available, the method can be performed through visual detection of a titration endpoint, which does not enable an analysis of colored samples. Here, we developed a method with spectrophotometric detection of a titration endpoint, appropriate for moisture determination of colored samples. The reaction takes place in a sealed 4 ml cuvette. Detection is performed at 520 nm. Titration endpoint is determined from the graph of absorbance plotted against titration volume. The method has appropriate reproducibility (RSD = 4.3%), accuracy, an