role of camp signaling in the survival and infectivity of the protozoan parasite, leishmania donovani营信号在原生动物的生存和传染性寄生虫,杜氏利什曼虫.pdfVIP

SAGE-Hindawi Access to Research Molecular Biology International Volume 2011, Article ID 782971, 9 pages doi:10.4061/2011/782971 Review Article Role of cAMP Signaling in the Survival and Infectivity of the Protozoan Parasite, Leishmania donovani Arunima Biswas,1 Arijit Bhattacharya,2 and Pijush K. Das1 1 Molecular Cell Biology Laboratory, Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Kolkata 700032, India 2 Department of Biotechnology, Presidency College, Kolkata 700073, India Correspondence should be addressed to Pijush K. Das, pijushdas@iicb.res.in Received 31 January 2011; Accepted 1 April 2011 Academic Editor: Hemanta K. Majumder Copyright © 2011 Arunima Biswas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Leishmania donovani, while invading macrophages, encounters striking shift in temperature and pH (from 22◦C and pH 7.2 to 37◦C and pH 5.5), which act as the key environmental trigger for differentiation, and increases cAMP level and cAMP-mediated responses. For comprehensive understanding of cAMP signaling, we studied the enzymes related to cAMP metabolism. A stage- specific and developmentally regulated isoform of receptor adenylate cyclase (LdRACA) showed to regulate differentiation-coupled induction of cAMP. The soluble acidocalcisomal pyrophosphatase, Ldvsp1, was the major isoform regulating cAMP level in association with LdRACA. A differentially expressed soluble cytosolic cAMP phosphodiesterase (LdPDEA) might be related to infection establishment by shifting trypanothione pool utilization bias toward antioxidant de