spectrofluorometric analysis of new-generation antidepressant drugs in pharmaceutical formulations, human urine, and plasma samplesspectrofluorometric分析新一代抗抑郁药物在制药配方,人类尿液和血浆样本.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 2, Pages 59–71 doi:10.1155/2012/567207 Spectrofluorometric Analysis of New-Generation Antidepressant Drugs in Pharmaceutical Formulations, Human Urine, and Plasma Samples Ruchita S. Das and Y. K. Agrawal Institute of Research Development, Gujarat Forensic Sciences University, Sector 18-A, Gandhinagar 382007, India Correspondence should be addressed to Ruchita S. Das, das ruchita@ Copyright © 2012 R. S. Das and Y. K. Agrawal. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. Spectrofluorometric analysis for the quantification of new-generation antidepressant drugs belong to the class of selective serotonin reuptake inhibitors and selective norepinephrine reuptake inhibitors is described. Five different drugs, sertraline, paroxetine, citalopram, venlafaxine, and fluoxetine, were analysed for spectrofluorometric detection in pharmaceutical formulations and urine and plasma samples. The calibration curves were found linear between fluorescence intensity and drugs concentration in the range of 5–500 ng/μL with coefficients of determination above 0.9985 for all the analytes. The method recoveries were higher than 85% in all the three matrices. The intra- and interday variation coefficients were observed less than 8% and 13%, respectively. The limits of detection (LOD) and limit of quantification (LOQ) were found in the range of 0.016–0.084 ng/μL and 0.090–0.285 ng/μL, respectively. Additionally, the results were compared statistically for each analyte in all the three matrices and were found equivalent, which signifies the absence of matrix effect. Thus, the method will be applied successfully to the determination of the cited drug