钙敏感受体在位脊髓损伤大鼠心肌中的表达.pdfVIP

钙敏感受体在高位脊髓损伤大鼠心肌中的表达 中文摘要 目的：探讨钙敏感受体在高位脊髓损伤大鼠心肌细胞中的表达。 方法：健康雄性SD 大鼠30 只，随机分为五组（n=6 ）：假手术组（S 组）、高位 脊髓损伤后6h （A 组）、12h (B 组) 、24h(C 组) 、48h(D 组) 。戊巴比妥麻醉后， 固定大鼠，咬除C6-T1 棘突及椎板，显露其下方硬膜，用Allens 打击法，建立 高位脊髓损伤大鼠模型。分别于6h、12h、24h 和48h 时点测定血清肌酸激酶(CK)、 肌酸激酶同工酶(CK-MB)和肌钙蛋白I(cTnI)含量；荧光定量PCR 检测心肌组织 CaSR mRNA 表达变化；Western blot 检测心肌组织CaSR 蛋白变化；透射电镜下 观察心肌细胞的超微结构特征。 结果：与假手术组比较，高位脊髓损伤大鼠各组cTnI、CK、CK-MB 均显著升高 (P0.05)，其中cTnI 和CK-MB 在12h 达到峰值，CK 在24h 达到峰值。钙敏感 受体mRNA 和蛋白质表达显著性升高（P0.05 ），并且24h 达到高峰，透射电镜 下可见大鼠心肌肌丝灶样溶解，线粒体空泡变性，心肌细胞凋亡。 结论：高位脊髓损伤后短时间内大鼠血清cTnI、CK、CK-MB 显著性升高，提示 有心肌损伤，且48h 不能恢复；高位脊髓损伤后心肌细胞在电镜下表现出随着时 间延长出现心肌细胞损伤加重，可见心肌细胞凋亡表现，12h 最明显；高位脊髓 损伤后CasR 出现过度表达，此可能是SCI 后心肌损伤的重要机制之一 关键词：高位脊髓损伤；钙敏感受体；心肌损伤 4 The Expression of calcium sensing receptor during myocardial damage by high-level spinal cord injury in rats Abstact Objection :Expression of calcium sensing receptor(CaSR) during myocardial damage by high-level spinal cord injury(SCI) in rats to observe the mechanism of myocardial damage by spinal cord injury in rats. Methods:The experimental model was established by high-level spinal cord injury in rats.SD rats were randomly divided into 5 groups:sham group,spinal cord injury 6h,12h,24h,48h groups(A,B,C,D group).The level of plasma creatine kinase(CK), MB isoenzyme of creatine kinase(CK-MB) and troponin Ⅰ(cTnI)were measured.CaSR mRNA expression was detected by fluorescence quantitative PCR. CaSR protein expression was detected by Western blot. The change of ultrastructure in the myocardium of rats was observed by electron microscopy. Result: In 4 ？injury groups, the values of cardiac enzymes were much higher than that of sham group(p0.05) and the expression of