hplc determination of the content of lipids capsules emodin（高效液相色谱法测定脂质胶囊大黄素的含量）.docVIP

HPLC determination of the content of lipids Capsules emodin Abstract: The purpose of quality of lipids Ling Capsule control of the new method. Methods: a C18 column, the mobile phase was methanol: 0.1% phosphoric acid solvent (85:15, detection wavelength of 254nm. Results: emodin (C15H10O5 control the 0.0565- good linear relationship 0.1695μg range, with an average recovery rate was 97.69%, RSD was 1.64%. Conclusions: The method is simple, sensitive, accurate, quality control methods can be used as the lipid Ling Capsule. Keywords: lipid Ling Capsule emodin high-performance liquid chromatography. 1, An instrument and reagent 1.1, instrumentation, high-performance liquid chromatograph (Shimadzu Corporation: LC-10AT pump, SPD-10A UV detector, CTO-10S oven, HW chromatography workstation. 1.2 drugs: blood lipids Ling Capsules: Capsules 0.45g pilot production of Wuhu City boing Pharmaceutical Co., Ltd., specifications, batch number 090 812 090 813 090 814 1.3, emodin reference substance (China Pharmaceutical and Biological Products, batch number :110756 -200,110 for content determination Reagents used in the experiment were HPLC grade or analytical grade. 2 Methods and Results 2.1, chromatographic conditions with reference to the 2005 edition Pharmacopoeia lipids Tablets [Determination] under chromatographic conditions determine Column: Shim-pack VP-ODS C18 column (150 × 4.6 mm, 5 μg, mobile phase: methanol: 0.1% phosphoric acid solution (85:15, flow rate: 0.8ml/min, detection wavelength: 254nm, column temperature: 30 ℃, injection volume: 20 μl. 2.2, system suitability test 2.2.1, the number of theoretical plates (N Take the right amount of emodin reference solution, into the liquid chromatograph and measured results emodin reference substance N = 7228, therefore, the number of theoretical plates is tentatively scheduled for 3000. 2.2.2 separation (R Take the test solution is the right amount, and injected into the liqu