expression of granzyme b gene in hela cells（在海拉细胞granzyme b基因的表达）.docVIP

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expression of granzyme b gene in hela cells（在海拉细胞granzyme b基因的表达）.doc

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expression of granzyme b gene in hela cells（在海拉细胞granzyme b基因的表达）

Expression of granzyme B gene in HeLa cells Papers to write network: Author: Zhao Jing Xu Yanming Jia Lintao in Cui-Juan Chen Lei Huang authoritarian Wang Ji Young Anyang Iron and Steel [Keywords] Gene Expression Keywords: granzyme B; gene expression; HeLa cells Abstract: Objective To construct the eukaryotic expression vector carrying human granzyme B gene and its expression in HeLa cells. Reverse transcription PCR for human granzyme B full-length cDNA sequences, the active type sequences were cloned into the reorganization of pcD-NA3 expression plasmid liposome method transfected HeLa cells, indirect immunofluorescence detection of the expression of the target protein, HE staining transfected HeLa cell morphology. Results of the wild type granzyme B cDNA construct encodes an active granzyme B the eukaryotic expression vector pcDNA3-G. to the target protein is observed in the transfected HeLa cells transfected cell morphology change, multinucleated giant cells and solid narrow cells Conclusion active-type granzyme B mammalian expression system for further The biological effects of the research granzyme B laid the foundation. Keywords: granzyme B; gene expression; HeLa cells Abstract: AIM To construct eukaryotic expression vector carrying human granzyme B gene and express it in HeLa cells.METHODS RT-PCR was used to obtain human granzyme B cDNA.Then the recombinant expression vector pcDNA3-G was constructed by cloning active ganzyme B into eukaryotic expression vector pcDNA3.After transfection into HeLa cells through lipofectamine2000 TM, the expression of target gene was detected by indirect immunofluorescence as-say and its effect on HeLa cells was observed by HE staining.RESULTS The obtained human granzyme B cDNA was con-sistent with that in GenBank.The eukaryotic expression vec-tor pcDNA3 -G encoding active granzyme B was successfully constructed and expressed in HeLa cells.Some transfected cells grew bigger than the control, often with multip