expression of nuclear factor-κb in primary cultured neurons ischemia-reperfusion（表达核factor-κb主要培养神经元缺血再灌注）.docVIP

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expression of nuclear factor-κb in primary cultured neurons ischemia-reperfusion（表达核factor-κb主要培养神经元缺血再灌注）.doc

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expression of nuclear factor-κb in primary cultured neurons ischemia-reperfusion（表达核factor-κb主要培养神经元缺血再灌注）

Expression of nuclear factor-κB in primary cultured neurons ischemia-reperfusion : Tian Ye, Wan Qi, Liu Yong, Li Li [Words] reperfusion injury [Abstract] AIM: To explore the expression of nuclear factor NFκB in primary cultured mouse fetus neurons. METHODS: The neurons primarily cultured in vitro after ischemialike reperfusion, and the expression of NFκB was assayed by immunofluorescence and flow cytometry. RESULTS: Immunofluorescence results showed that the expression of NFκB increased and moved from cytoplasm to nuclear. Flow cytometry results showed that the expression of NFκB was 1.28% (before ischemiareperfusion) and 38.72% (after ischemiareperfusion). CONCLUSION: NFκB exits in neural cells and after ischemiareperfusion injury it will be activated, move from cytoplasma to nuclei and play a regulatory role of gene transcription. [Keywords] NFκB; neuron; reperfusion injury [Abstract] Objective: To observe the nuclear factor kappa B (nuclearfactorκB of NFκB expression in fetal rat primary cultured neurons and transfer and discuss its significance. METHODS: Cultured primary cultured neurons by class ischemia treatment after reoxygenation with immunofluorescence staining, flow cytometry NFκB expression. Results: Immunofluorescence antibody staining after ischemia-reperfusion NFκB expression increased by the cytoplasm into the nucleus, flow cytometry shows ischemia-reperfusion the NFκB expression before and after were 1.3% and 38.7%, there is a significant difference (P lt;0.01. Conclusions: NFκB exists in nerve cells after ischemia-reperfusion NFκB is activated by the cytoplasm into the nucleus, to play its gene regulatory role. [Words] of nuclear factor kappa B neurons reperfusion injury 0 Introduction Nuclear factor kappa B (nuclearfactorκB, NFκB is an important transcription factor exists in eukaryotic cells, involved in the regulation of expression of many genes. Resting state, NFκB and inhibitory protein (inhibitorκB, the I kappa B form