氢醌对体外培养骨髓基质细胞核转录因子表达影响.docVIP

氢醌对体外培养骨髓基质细胞核转录因子表达影响 　 作者：杨海玉，杨建国，王光汉，俞康 【摘要】 　　为了研究氢醌(hydroquinone，HQ)对细胞激活剂佛波酯（PMA）导致的骨髓基质细胞（BMSC）核转录因子表达的影响，并初步探讨其与苯的血液学毒性的关系。用体外培养法获得骨髓基质细胞，观察其形态学变化；分别用NF-κB P65免疫组织化学方法和TransAM P65试剂盒测定了用不同浓度的氢醌加激活剂PMA刺激后的骨髓基质细胞NF-κB蛋白表达和活性的动态变化。结果表明：各组细胞加入HQ后，与对照组相比，P65蛋白由细胞核转回至胞核周围的细胞浆，染色呈弱阳性；不同浓度的HQ作用不同时间后NF-κB活性测定结果与对照组间具有明显差异；各组之间相比，100 μmol/L的HQ作用72小时对NF-κB的抑制作用最明显；而0.1 μmol/L的HQ几乎无抑制作用。结论： HQ可抑制体外培养骨髓基质细胞核转录因子的激活，并且随HQ浓度和作用时间而增强，推测可能与苯的造血微环境毒性有关。 【关键词】 基质细胞;体外培养;氢醌;核转录因子 　　Hydroquinone Inhibits NF-κB Expression in Human Bone Marrow Stromal Cells In Vitro 　　Abstract This study was aimed to investigate whether hydroquinone (HQ) can inhibit NF-κB expression activated by phorbol myristate acetate (PMA)，and to explore the relationship between the mechanism and the hematology toxicity of benzene tentatively. The human bone marrow stromal cells (BMSC) were harvested by in vitro culture and their change of morphology were observed. The activity and protein expression of NF-κB p65 extracted from those BMSC were measured with immunohistochemistry and TransAM P65 kit. The results showed that in cells exposed to HQ，P65 transferred from cell nucleus to cytoplasma around cell nucleus and its concentration lowered by immunohistochemistry. And TransAM P65 kit assay revealed that HQ effects at different concentrations were distinctive at respective time.The detected parameters in 100 μmol/L HQ group were significantly different from control group after exposure for 72 hours. But the parameters at different time in μmol/L HQ group were not obviously different. It is concluded that hydroquinone can inhibit NF-κB activated by PMA in BMSCs culture. This kind of inhibitory action correlated with the concentration of HQ and exposure time. 　　Key words stromal cell; culture in vitro; hydroquinone; NF-κB 苯是一种应用广泛的溶剂和化工原料［1-5］，目前还不能停止它的使用；但另一方面，苯也是一种主要的环境污染物和毒物。苯对血液系统的影响主要表现为抑制骨髓的造血功能，导致血液系统病变，抑制基质细胞的生长，并通过干扰骨髓基质细胞分泌造血生长因子的能力，抑制骨髓的造血功能，导致血液系统病变，如贫血、再生障碍性贫血、骨髓增生异常综合症（MDS）、白血病等。到目前为止，苯诱发再生障碍性贫血