炮制工艺对白芍中芍药苷影响.docVIP

炮制工艺对白芍中芍药苷影响 　 作者：李云峰 房敏峰 张文娟 郑晓晖 王亚洲 曲欢欢 毕业论文 【摘要】 　　目的 考察亳白芍、杭白芍和川白芍及其不同炮制品中芍药苷的含量变化，为 研究 不同炮制工艺对白芍临床作用的 影响 提供依据。 方法 采用RP-HPLC法测定三种不同规格白芍及其相应四种炮制品中芍药苷的含量，色谱柱：SHIMADZU VP-ODS（150mm×4.6mm，5μm）；流动相：乙腈-0.1％磷酸水溶液（16：84）；流速：1.0ml/min；柱温：25℃；检测波长：230nm。结果 三种不同规格药材中芍药苷的含量为：亳白芍、杭白芍和川白芍分别为3.30%、3.14%和2.50%；白芍药材、白芍片、酒白芍、炒白芍和醋白芍中芍药苷的含量依次为2.98%、2.73%、2.69%、2.50%、2.48%。结论 芍药苷（C23H28O11）含量：亳白芍gt;杭白芍gt;川白芍；药材gt;白芍片≈酒白芍gt;炒白芍≈醋白芍。 毕业论文 【关键词】 白芍 炮制 芍药苷 　　 The influence of unprocessed and processed products of Radix Paeoniae Alba 【Abstract】 Objective To provide the foundation for studying the clinical effect about processsing art work of Radix Paeoniae Alba, which based on the contents of peoniflorin in unprocessed and processed products of Radix Paeoniae Alba. Methods Using the reversed phase HPLC system to determine the contents of peoniflorin in three different specification of unprocessed and four processed products of Radix Paeoniae Alba.The chromatographic column is SHIMADZU VP-ODS（150mm×4.6mm,5μm）column;the mobile phase is acetonitrile-0.05%phosphoric acid（16：84;flow rate:1.0ml/min,column temperature:25℃;detection wave lenth:230nm）. Results The contents of peoniflorin from Bozhou、Hangzhou、Sichuan were 3.30%、3.14% and 2.50%,and Radix Paeoniae Alba、Radix Paeoniae Alba slice、stir-baked radix paeoniae alba with vino、stir-baked Radix Paeoniae Alba and stir-baked Radix Paeoniae Alba with vinegar were 2.98%,2.73%,2.69%,2.50%,2.48%.Conclusion Calculated by dry character,peoniflorin（C23H28O11）,Radix Paeoniae Alba from Bozhougt;Radix Paeoniae Alba from Hangzhou ≈Radix Paeoniae Alba from Sichuan;Radix Paeoniae Albagt;Radix Paeoniae Alba slice≈stir-baked Radix Paeoniae Alba with vinogt;stir-baked Radix Paeoniae Alba≈stir-baked Radix Paeoniae Alba with vinegar;The changes of transfer rates and peoniflorin in different processed products of Radix Paeoniae Alba were in coincidence. 【Key words】 Radix Paeoniae Alba; processing; peoniflorin 论文代写 白芍为毛茛科植物芍药Paeunia lac