high molecular weight glucan of the culinary medicinal mushroom agaricus bisporus is an α-glucan that forms complexes with low molecular weight galactan高分子量葡聚糖的烹饪药用蘑菇双孢菇的α-glucan形式与低分子量半乳糖体复合物.pdfVIP
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high molecular weight glucan of the culinary medicinal mushroom agaricus bisporus is an α-glucan that forms complexes with low molecular weight galactan高分子量葡聚糖的烹饪药用蘑菇双孢菇的α-glucan形式与低分子量半乳糖体复合物
Molecules 2010, 15, 5818-5830; doi:10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
High Molecular Weight Glucan of the Culinary Medicinal
Mushroom Agaricus bisporus is an α-Glucan that Forms
Complexes with Low Molecular Weight Galactan
Fhernanda R. Smiderle 1,2, Guilherme L. Sassaki 2, Jeroen van Arkel 1, Marcello Iacomini 2,
Harry J. Wichers 3 and Leo J.L.D. Van Griensven 1,*
1 Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1,
6708 PB Wageningen, Netherlands
2 Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Paraná, Centro
Politécnico, CP 19046, Curitiba-PR, Brazil
3 Wageningen University and Research Centre, Bornse Weilanden 9, 6708 WG Wageningen,
Netherlands
* Author to whom correspondence should be addressed; E-Mail: leo.vangriensven@wur.nl;
Tel.:+31 317480992; Fax: +31 317418094.
Received: 16 July 2010; in revised form: 20 August 2010 / Accepted: 24 August 2010 /
Published: 25 August 2010
Abstract: An -glucan was isolated from the culinary medicinal mushroom A. bisporus by
hot water extraction, ethanol precipitation and DEAE-cellulose chromatography. The
resulting material showed a single HMW peak excluded from a Sephadex G50 column that
could completely be degraded by α-amylase treatment. After heating in 1% SDS a small
additional peak of low MW eluted from the G50 column. The monosaccharide composition
of the main peak was evaluated by HPLC, and was found to consist of a majority o
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