原创力文档networking of differentially expressed genes in human cancer cells resistant to methotrexate网络在人类癌症细胞的差异表达基因的抗甲氨蝶呤.pdfVIP
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networking of differentially expressed genes in human cancer cells resistant to methotrexate网络在人类癌症细胞的差异表达基因的抗甲氨蝶呤
Research
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Elisabet Selga, Carlota Oleaga, Sara Ramírez, M Cristina de Almagro,
Véronique Noé and Carlos J Ciudad
Address: Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Diagonal Avenue,
E-08028 Barcelona, Spain.
Correspondence: Carlos J Ciudad. Email: cciudad@
Published: 4 September 2009 Received: 22 May 2009
Revised: 31 July 2009
Genome Medicine 2009, 11::83 (doi:10.1186/gm83)
Accepted: 4 September 2009
The electronic version of this article is the complete one and can be
found online at /content/1/9/83
© 2009 Selga et al.; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
AAbbssttrraacctt
BBaacckkggrroouunndd:: The need for an integrated view of data obtained from high-throughput technologies
gave rise to network analyses. These are especially useful to rationalize how external
perturbations propagate through the expression of genes. To address this issue in the case of
drug resistance, we constructed biological association networks of genes differentially expressed
in cell lines resistant to methotrexate (MTX).
MMeetthhooddss:: Seven cell lines representative of different types of cancer, including colon c
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