原创力文档non invasive in vivo investigation of hepatobiliary structure and function in stii medaka (oryzias latipes) methodology and applications体内非侵入性的调查肝胆管的结构和功能在stii青鳉(oryzias latipes)方法和应用.pdfVIP
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non invasive in vivo investigation of hepatobiliary structure and function in stii medaka (oryzias latipes) methodology and applications体内非侵入性的调查肝胆管的结构和功能在stii青鳉(oryzias latipes)方法和应用
Comparative Hepatology BioMed Central
Research Open Access
Non invasive in vivo investigation of hepatobiliary structure and
function in STII medaka (Oryzias latipes): methodology and
applications
Ron C Hardman*, Seth W Kullman and David E Hinton
Address: Duke University, Environmental Sciences and Policy Division, Nicholas School of the Environment and Earth Sciences, LSRC A333,
Durham NC, USA
Email: Ron C Hardman* - ron.hardman@; Seth W Kullman - swkull@; David E Hinton - dhinton@
* Corresponding author
Published: 6 October 2008 Received: 20 March 2007
Accepted: 6 October 2008
Comparative Hepatology 2008, 7:7 doi:10.1186/1476-5926-7-7
This article is available from: /content/7/1/7
© 2008 Hardman et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: A novel transparent stock of medaka (Oryzias latipes; STII), recessive for all pigments
found in chromatophores, permits transcutaneous imaging of internal organs and tissues in living
individuals. Findings presented describe the development of methodologies for non invasive in vivo
investigation in STII medaka, and the successful application of these methodologies to in vivo study
of hepatobiliary structure, function, and xenobiotic response, in both 2 and 3 dimensions.
Results: Using
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