quantitative proteomics for identifying biomarkers for tuberculous meningitis定量蛋白质组学为结核性脑膜炎标志物.pdfVIP

Kumar et al. Clinical Proteomics 2012, 9:12 /content/9/1/12 CLINICAL PROTEOMICS RESEARCH Open Access Quantitative proteomics for identifying biomarkers for tuberculous meningitis 1,2 1,2,3,4 5 1,3,4,6 1 Ghantasala S Sameer Kumar , Abhilash K Venugopal , Anita Mahadevan , Santosh Renuse , H C Harsha , 1,7 1,8 9 1 10 Nandini A Sahasrabuddhe , Harsh Pawar , Rakesh Sharma , Praveen Kumar , Sudha Rajagopalan , Keith Waddell11 2 12 1,2,3,4 , Yarappa L Ramachandra , Parthasarathy Satishchandra , Raghothama Chaerkady , T S Keshava Prasad 1,6,7,13, K Shankar5* and Akhilesh Pandey1,3,4,14,15* Abstract Introduction: Tuberculous meningitis is a frequent extrapulmonary disease caused by Mycobacterium tuberculosis and is associated with high mortality rates and severe neurological sequelae. In an earlier study employing DNA microarrays, we had identified genes that were differentially expressed at the transcript level in human brain tissue from cases of tuberculous meningitis. In the current study, we used a quantitative proteomics approach to discover protein biomarkers for tuberculous meningitis. Methods: To compare brain tissues from confirmed cased of tuberculous meningitis with uninfected brain