synthesis and characterization of anti-her2 antibody conjugated cdsecdzns quantum dots for fluorescence imaging of breast cancer cells合成和表征anti-her2抗体共轭cdsecdzns量子点荧光成像的乳腺癌细胞.pdfVIP

Sensors 2009, 9, 9332-9354; doi:10.3390/ OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe/CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cells 1 2 2 1 Dhermendra K. Tiwari , Shin-Ichi Tanaka , Yasushi Inouye , Keiko Yoshizawa , Tomonobu M. Watanabe 1 and Takashi Jin 1,2,* 1 WPI Immunology Frontier Research Center, Osaka University, Yamada-oka 1-3, Suita, Osaka 565- 0871, Japan; E-Mails: mdkdragon@ (D.T.); kyoshizawa@fbs.osaka-u.ac.jp (K.Y.); twatanabemeister@ (T.W.) 2 Graduate School of Frontier Biosciences, Osaka University, Yamada-oka 2-1, Suita, Osaka 565- 0871, Japan; E-Mails: s-tanaka@ap.eng.osaka-u.ac.jp (S.T.); ya-inoue@ap.eng.osaka-u.ac.jp (Y.I.) * Author to whom correspondence should be addressed; E-Mail: jin@fbs.osaka-u.ac.jp; Tel.: +81-6-6879-4427; Fax: +81-6-6879-4426. Received: 21 September 2009; in revised form: 3 November 2009 / Accepted: 12 November 2009 / Published: 19 November 2009 Abstract: The early detection of HER2 (human epidermal growth factor receptor 2) status in breast cancer patients is very important for the effective implementation of anti-HER2 antibody therapy. Recently, HER2 detections using antibody conjugated quantum dots (QDs) have attracted much attention. QDs are a new class of fluorescent materials that have superior properties such as high brightnes