the antiangiogenic compound aeroplysinin-1 induces apoptosis in endothelial cells by activating the mitochondrial pathway抗血管新生化合物aeroplysinin-1激活内皮细胞发生凋亡的线粒体途径.pdfVIP

Mar. Drugs 2012, 10, 2033-2046; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article The Antiangiogenic Compound Aeroplysinin-1 Induces Apoptosis in Endothelial Cells by Activating the Mitochondrial Pathway Beatriz Martínez-Poveda 1, Salvador Rodríguez-Nieto 2, Melissa García-Caballero 1, Miguel-Ángel Medina 1,3 and Ana R. Quesada 1,3,* 1 Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, Málaga E-29071, Spain; E-Mails: bmpoveda@ (B.M.-P.); melissa@uma.es (M.G.-C.); medina@uma.es (M.-A.M.) 2 Genes and Cancer Group, Cancer Epigenetics and Biology Program (PEBC-IDIBELL), Barcelona E-08908, Spain; E-Mail: srodriguezn@idibell.cat 3 Centre for Biomedical Network Research on Rare Diseases (CIBERER), Málaga E-29071, Spain * Author to whom correspondence should be addressed; E-Mail: quesada@uma.es; Tel.: +34-952-137-128; Fax: +34-952-132-000. Received: 18 July 2012; in revised form: 3 September 2012 / Accepted: 6 September 2012 / Published: 18 September 2012 Abstract: Aeroplysinin-1 is a brominated metabolite extracted from the marine sponge Aplysina aerophoba that has been previously characterized by our group as a potent antiangiogenic compound in vitro and in vivo. In this work, we provide evidence of a selective induction of apoptosis by aeroplysinin-1 in endothelial cells. Studies on the nuclear morphology of treated cells revealed that aeroplysinin-1 induces chromatin