原创力文档use of biotinylated ubiquitin for analysis of rat brain mitochondrial proteome and interactome使用生物素化的泛素的老鼠大脑线粒体蛋白质组分析和interactome.pdfVIP
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use of biotinylated ubiquitin for analysis of rat brain mitochondrial proteome and interactome使用生物素化的泛素的老鼠大脑线粒体蛋白质组分析和interactome
Int. J. Mol. Sci. 2012, 13, 11593-11609; doi:10.3390/ijms130911593
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Use of Biotinylated Ubiquitin for Analysis of Rat Brain
Mitochondrial Proteome and Interactome
1 2 1 1
Olga A. Buneeva , Marina V. Medvedeva , Arthur T. Kopylov , Victor G. Zgoda and
Alexei E. Medvedev 1,*
1 Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences,
10 Pogodinskaya street, Moscow 119121, Russia; E-Mails: olbun@yandex.ru (O.A.B.);
a.t.kopylov@ (A.T.K.); vic@ibmh.msk.su (V.G.Z.)
2 Moscow State University, Moscow, 119991, Russia; E-Mail: marmed64@yandex.ru (M.V.M.)
* Author to whom correspondence should be addressed; E-Mail: professor57@yandex.ru;
Tel.: +7-4992450509; Fax: +7-4992450857.
Received: 27 July 2012; in revised form: 22 August 2012 / Accepted: 3 September 2012 /
Published: 14 September 2012
Abstract: Applicability of in vitro biotinylated ubiquitin for evaluation of endogenous
ubiquitin conjugation and analysis of ubiquitin-associated protein-protein interactions has
been investigated. Incubation of rat brain mitochondria with biotinylated ubiquitin
followed by affinity chromatography on avidin-agarose, intensive washing, tryptic
dige
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