原创力文档use of oligonucleotides carrying photolabile groups for the control of the deposition of nanoparticles in surfaces and nanoparticle association使用携带对光不稳的寡核苷酸组沉积的控制纳米粒子的表面和纳米颗粒.pdfVIP
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use of oligonucleotides carrying photolabile groups for the control of the deposition of nanoparticles in surfaces and nanoparticle association使用携带对光不稳的寡核苷酸组沉积的控制纳米粒子的表面和纳米颗粒
Int. J. Mol. Sci. 2011, 12, 7238-7249; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Use of Oligonucleotides Carrying Photolabile Groups for the
Control of the Deposition of Nanoparticles in Surfaces and
Nanoparticle Association
Brendan Manning 1,2 and Ramon Eritja 1,2,*
1
Institute for Research in Biomedicine, Baldiri Reixac 10, Barcelona E-08028, Spain;
E-Mail: Brendan.manning@
2
Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, CIBER-BBN Networking Centre on
Bioengineering, Biomaterials and Nanomedicine, Jordi Girona 18-26, Barcelona E-08034, Spain
* Author to whom correspondence should be addressed; E-Mail: recgma@cid.csic.es;
Tel.: +34-93-403-9942; Fax: +34-93-204-5904.
Received: 10 October 2011 / Accepted: 18 October 2011 /Published: 24 October 2011
Abstract: An oligodeoxynucleotide hairpin containing a photolabile 2-nitrobenzyl group
in the loop and terminated with a thiol function was prepared. The photocleavage of such
a hairpin on gold yields a surface activated with a single stranded oligonucleotide which
can be utilised to direct the assembly of nanoparticles conjugated with a complementary
strand. Analysis of photocleaved surfaces gives nanoparticle coverage one order of
magnitude higher than nonphotocleaved surfaces. This illustrates the ability of
photocleavable hairpins to direct th
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