针刺对Ⅳ型变态反应模型豚鼠干预作用探究.docVIP

针刺对Ⅳ型变态反应模型豚鼠干预作用探究 　 作者：谢长才，许能贵，符文彬，孙健 【摘要】 　　【目的】观察针刺对Ⅳ型变态反应模型豚鼠的影响。【方法】选用白化豚鼠36只，随机分为3组：空白组、模型组、针刺组；后2组采用二硝基氯苯（DNCB）外用致敏及反复激发方法复制豚鼠Ⅳ型变态反应模型；针刺组电针曲池及血海穴，治疗10d后取材，观察耳厚度与质量、真皮内炎症细胞数及病理变化。【结果】模型组耳厚度和质量显著性升高（P＜001），单核细胞与淋巴细胞数、中性与嗜酸性粒细胞数显著升高（P＜005），并出现慢性湿疹的病理改变；针刺组可显著降低模型豚鼠耳厚度与质量（均P＜005），一定程度降低炎症细胞数（P＞005）,并可明显减轻皮肤慢性湿疹的病理改变。【结论】针刺具有一定的抗Ⅳ型变态反应作用。 【关键词】 变态反应/针灸疗法；皮肤/病理学；疾病模型，动物；豚鼠；穴，曲池；穴，血海 　　Abstract: ObjectiveTo observe the intervention of acupuncture on guinea pig models of allergic reaction type Ⅳ.MethodsThirtysix albino guinea pigs were randomized into blank control group，model group，and acupuncture group.The guinea pig models of allergic reaction type Ⅳ were established by external application of dinitrochlorobenzene.The acupuncture group received acupuncture on acupoints of Quchi and Xuehai for 10 days.After treatment，the auricular thickness and weight，the number of intradermal inflammatory cells，and the auricular pathological changes in guinea pigs were observed.Results In the model group，the auricular thickness and weight were increased，and the number of intradermal monocytes，lymphocytes，neutrophils，and fibrocytes was also increased (P＜005 or P＜001).Meanwhile，pathological changes of chronic eczema were shown in the model group.Compared with those in the model group，the auricular thickness and weight were decreased in acupuncture group (P＜005)，the number of inflammatory cells were lowered to some extent (P＞005)，and the pathological changes of chronic eczema were relieved.Conclusion Acupuncture exerts an intervention on allergic reaction type Ⅳ. 　　Key words: ALLERGIC REACTION /acupuncture amp; moxibustion therapy;SKIN/pathology; DISEASE MODELS，ANIMAL;GUINEA PIGS；POINT LI11(QUCHI); 　　Ⅳ型变态反应是由细胞介导的迟发性超敏反应所致的皮肤病，是常见的变应性炎症性皮肤病。其中最常见的有慢性湿疹和变态反应性接触性皮炎，临床特点表现为剧烈瘙痒，有多形性皮疹及渗出倾向，易于复发并趋向慢性化［1］。临床研究证实针灸治疗Ⅳ型变态反应性皮肤病，具有抗炎、止痒、调节机体免疫功能的作用［2］。本实验观察了针刺对Ⅳ型变态反应模型豚鼠的影响，为临床提供针刺治疗Ⅳ型变态反应的理论依据，现将结果报道如下。 　　1材料与方法 　　11动物及分组 　　普通级雄性白化豚鼠，体质量250～300g，由广州中医药大学实验动物中心提供。合格证编号：0021021，0