麝香保心丸对自发性高血压大鼠心脏保护作用探究.docVIP

麝香保心丸对自发性高血压大鼠心脏保护作用探究 　 作者：胡送友，田登科，陈刚领，刘俊，可燕，卞卡 【摘要】 　　目的探讨麝香保心丸（HMP）对自发性高血压大鼠（SHR）心脏保护作用及其可能的机制。方法将6 周龄SHR分为HMP治疗组、高血压模型组,将WKY大鼠作为正常血压组，每组分3批在3个时间点（给药6 周、给药14 周、停药9 周）处死大鼠，尾套法测定血压；FRAP法进行心脏组织总抗氧化能力测定；采用RT-PCR考察炎症相关因子ICAM-1、VCAM-1、IL-1β和TNFα mRNA表达；Western Blot与ELISA法分别考察炎症相关因子ICAM-1、VCAM-1及IL-1β、TNF α蛋白表达。结果HMP对SHR无明显降压作用，但能提高SHR心脏组织总抗氧化能力。PCR、Western Blot与ELISA显示给予HMP 6 周和14周后，均能显著抑制SHR心脏炎性相关因子ICAM-1、VCAM-1 m RNA表达及ICAM-1蛋白表达；给药14周后，能显著抑制炎性相关因子IL-1β、TNF α mRNA表达及蛋白表达；停药9周后，仍能抑制炎性相关因子ICAM-1、TNFαmRNA表达及ICAM-1蛋白表达。结论HMP具有独立降压以外的对SHR心脏保护作用，其机制可能与抑制心脏组织炎症反应及降低组织氧化应激有关。 【关键词】 麝香保心丸 自发性高血压大鼠 炎症 心脏 原发性高血压 　　Abstract：ObjectiveTo investigate the protective effect of Heart-protecting musk pill (HMP) on heart in spontaneously hypertensive rats (SHR), and its possible mechanisms. Methods6 wk-old male SHR were randomly dicided into the HMP treatment group and model. Age-matched male Wistar-Kyoto (WKY) normotensive rats were used as the control group. Rats in each group were periodically decapitated in batch at three time points (6 week-treatment, 14 week-treatment, and 9 week post-treatment).Systolic blood pressure(SBP) was measured by tail-cuff method. Total antioxidant capacity of heart was evaluated with FRAP method. RT-PCR was used to measure the mRNA expression of inflammation-related mediators (ICAM-1,VCAM-1,IL-1β and TNFα).The protein expression of inflammation-related mediators (ICAM-1,VCAM-1, IL-1β,TNFα) was determined by Western Blot and ELISA.ResultsHMP had no marked effect on SBP, but could elevate total antioxidant capacity of heart in SHR. PCR , Western Blot and ELISA showed that HMP could inhibit ICAM-1,VCAM-1 mRNA expression and ICAM-1 protein expression of heart in SHR after 6 weeks and 14 weeks of treatment, inhibit IL-1β,TNFα mRNA expression and protein expression after 14 weeks of treatment, also inhibit ICAM-1,TNFα mRNA expression and ICAM-1 protein expression after 9 weeks of post-treatment. ConclusionHMP has protective effect on heart independent of its