CD44v6及uPA 在胃癌组织中表达及相关性探究.docVIP

CD44v6及uPA 在胃癌组织中表达及相关性探究 　【摘要】 目的：观察CD44v6和uPA 在胃癌组织中的表达情况，分析它们在各种组织类型及不同分化程度胃癌中的表达规律,探讨它们的异常表达与胃癌侵袭转移的关系。方法：采用免疫组织化学SP 法分别检测CD44v6和uPA 在67 例胃癌组织中的表达。结果： （1）67 例胃癌组织中CD44v6阳性表达34 例(50.75%, uPA 阳性表达35例(52.24%)；（2）CD44v6、uPA 在低分化胃癌中的表达率均比高分化中的高；（3）有淋巴结转移者的CD44v6、uPA阳性率比无转移者显著增高；（4）早期胃癌组、侵及肌层组和侵犯全层组CD44v6阳性表达率分别为7.69%、45.00%、70.59%，差异有统计学意义(Plt;0.01)。uPA的阴性表达率也有统计学意义；（5）胃癌中CD44v6 和uPA 的表达呈正相关(Plt;0.01) 。结论：CD44v6、uPA 的表达均与胃癌的分化程度、浸润深度和淋巴结转移有关,二者在胃癌的浸润和转移过程中可能有协同作用。 【关键词】 胃癌;细胞表面整合蛋白-6;尿激酶型纤溶酶原激活物;浸润;转移 [Abstract] Objective：To study the expression of urokinase-type plasminogen activator(uPA) and cluster of differentiation 44-6 on the infiltration and metastasis of gastric cancer and to investigate the relationship of their expression. Methods：Immunohistochemical technique (S-P method) was applied to detect CD44v6 and uPA in 67 cases of gastric cancer. Results：（1）Of the 67 cases gastric cancer, 34(50.75 %) were positive for CD44v6 , 35 (52.24%) for uPA. The co-expression of CD44v6 and uPA was 26(38.81%)；（2）The CD44v6 positive rate in well differentiated carcinoma was higher than that in poorly one (Plt;0.05) ; The same result was concluded about uPA（Plt;0.01）；（3）CD44v6 and uPA positive rates in the cases with lymph node metastasis were relatively higher than that without lymph node metastasis（Plt;0.01）；（4）The differences of CD44v6 and uPA positive rates were significant among cases of early gastric cancer and cases with muscularis involvement and cases with whole layer infiltration(Plt;0.01)；（5）The relationship was significant between the expression of CD44v6 and uPA in gastric cancer (Plt;0.01) . Conclusion：The study shows that CD44v6 and uPA may play an important role in histologic types or invasion or metastasis of gastric cancer. The assay of CD44v6 and uPA in patients with gastric cancer , as the clinical valuable marker, has the active role and instructive significance in estimating prognosis and working out rational therapy. [Key words] Gastric cancer; CD44v6