CO2气腹对正常大鼠脑组织S100、 NSE mRNA及蛋白水平影响.doc

CO2气腹对正常大鼠脑组织S100、 NSE mRNA及蛋白水平影响 　【摘要】 目的 研究在不同CO2气腹压及不同时段下大鼠血和脑脊液S100蛋白(S100)、神经元特异性烯醇化酶(NSE)水平的变化对中枢神经系统的影响。方法 Wistar大鼠建立气腹模型，光镜和电镜观察大鼠脑组织胶质细胞和神经元细胞组织学变化；抗体夹心法检测血液和脑脊液中S100、NSE水平变化，RTPCR技术和免疫组织化学的方法观察脑组织中S100、NSE mRNA和蛋白水平表达的变化。结果CO2气腹后，A51组与对照组比较，海马区胶质细胞和神经元细胞均无明显变化，A102组胶质细胞和神经元细胞在光镜下呈轻度水样变，电镜下线粒体轻度肿胀；血液和脑脊液中S100、NSE在CO2气腹压力后逐渐增加，但差异无统计学意义(P＞0.05)，S100和NSE在血液和脑脊液中水平变化分别呈正相关(P＜0.05)；S100、NSE mRNA在CO2气腹后逐渐增加，但与对照组比较差异无统计学意(P＞0.05)；脑组织S100、NSE表达阳性面积随气腹时间及压力增加而逐渐增加，在A51组和A102组与对照组比较差异无统计学意义(P＞0.05)。结论 在10 mm Hg 2 h范围，CO2气腹对正常大鼠中枢神经系统无损害，CO2气腹在这一范围内应用对中枢神经系统是安全的。 【关键词】 S100蛋白 神经元特异性烯醇化酶 大鼠 CO2气腹 【Abstract】 Objective To evaluate the changes of S100 protein (S100) and neuron specific enolase (NSE) of blood and cerebrospinal fluid (CSF) at different time and pressure of carbon dioxide pneumoperitoneun (CO2 PP) and explore the effect of CO2 PP on central nervous system (CNS) in normal rats. Methods Model of CO2 PP in Wistar rats was established to observe astrocytes and neurons in the hippocamps of brian tissue of rat by light microscope and electron microscope. The changes of S100 and NSE in blood and CSF were measured by ELISA. The mRNA and protein expressions of S100 and NSE were tested by RTPCR and immunohistochemical assay. Results There was no histologic change of hippocampus astrocytes and neurons between A51 group and control group after CO2 PP. The astrocytes and neurons in A102 group showed local hydropic degeneration under light microscope and mitochondrion swelling under electron microscope. The levels of S100 and NSE were increased in both blood and CSF samples in A51 group and A102 group after CO2 PP, without statistical difference (P＞0.05). The levels of S100 and NSE in the blood were found to be positively correlated with those in the CSF (P＜0.05). The mRNA expressions of S100 and NSE increased progressively in A51 group and A102 group, without statistical difference compared with control group (P＞0.05). The positive expre