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- 约 51页
- 2017-09-18 发布于江苏
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stemcell
to theneural
analyze proteome
Resu|ts:
canbe invitroin
1.TheNSCsisolatedfrom ratcortex
postnatal grown neurospheres.
Cultured were to BrdUandnestin.The
immunopositive
neurospheres
havethe todifferentiateinto and
neurospheresability neurons,astrocytes
afterinduction.
ollgodendroglias
dereasedDAPTtreatmentrecealed
2.Gene ofHeslandRBP-JK after
expression
could theNotch
that200nmol/LDAPTinhibit signalpathway.
resultof andCCK一8revealedthat ofNSCsWaS
3.The cytometry proliferation
inhibitedDAPTtreatment.
by
4.DAPTtreatmentthedifferentiationtrendofNSCs.Theresult
changed suggested
of differentiationofratneuralstemcellsshifc
thataftertheinduction
DAPT,the
the into
towardneuronsand differentiation
oligodendrocytes,while astrocyrtes
wasdecreased..
twodimensional and 2D software
Imagemaster
5.Applying electrophoresis platinum
to incontrolandtreatedand thatthe
levels investigate
compareproteinexpression
of11 isdifferentbetweenthecontrolandthetreated.The
protein
expression
identifiedmass involved forWnt
protein by spectrometryreceptor proteins,Cell
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