γ-分泌酶抑制剂DAPT对神经干细胞增殖分化的影响及可能机制.pdfVIP

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  • 2017-09-18 发布于江苏
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γ-分泌酶抑制剂DAPT对神经干细胞增殖分化的影响及可能机制.pdf

stemcell to theneural analyze proteome Resu|ts: canbe invitroin 1.TheNSCsisolatedfrom ratcortex postnatal grown neurospheres. Cultured were to BrdUandnestin.The immunopositive neurospheres havethe todifferentiateinto and neurospheresability neurons,astrocytes afterinduction. ollgodendroglias dereasedDAPTtreatmentrecealed 2.Gene ofHeslandRBP-JK after expression could theNotch that200nmol/LDAPTinhibit signalpathway. resultof andCCK一8revealedthat ofNSCsWaS 3.The cytometry proliferation inhibitedDAPTtreatment. by 4.DAPTtreatmentthedifferentiationtrendofNSCs.Theresult changed suggested of differentiationofratneuralstemcellsshifc thataftertheinduction DAPT,the the into towardneuronsand differentiation oligodendrocytes,while astrocyrtes wasdecreased.. twodimensional and 2D software Imagemaster 5.Applying electrophoresis platinum to incontrolandtreatedand thatthe levels investigate compareproteinexpression of11 isdifferentbetweenthecontrolandthetreated.The protein expression identifiedmass involved forWnt protein by spectrometryreceptor proteins,Cell

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