活血潜阳方对自发性高血压大鼠心肌肥厚组织原癌基因cfos及cm.docVIP

活血潜阳方对自发性高血压大鼠心肌肥厚组织原癌基因cfos及cm 　 作者：符德玉, 王世红, 周端, 马宇滢, 金露, 祖亮华 【摘要】 目的：通过分析左心室心肌原癌基因cfos和cmyc mRNA及蛋白的表达，探讨活血潜阳方改善自发性高血压大鼠（spontaneous hypertensive rat, SHR）左心室肥厚的作用机制。方法：以10周龄的SHR为高血压左心室肥厚模型，随机分为SHR模型组，中药大、中、小剂量治疗组，西拉普利治疗组，年龄和性别相匹配的京都（WistarKyoto, WKY）大鼠作为正常对照组，每组5只。灌胃14周后，取大鼠心脏组织，用原位杂交组织化学法和免疫组化法分别检测心肌cfos和cmyc mRNA及蛋白的表达。结果：24周龄模型组SHR左心室肥厚心肌的原癌基因cfos和cmyc mRNA及蛋白的表达与WKY大鼠比较明显增强（Plt;0.01）。与SHR模型组比较，大、中和小剂量活血潜阳方组大鼠左心室心肌组织中cfos和cmyc mRNA的表达下降（Plt;0.05）；大、中剂量活血潜阳方可降低cmyc蛋白的表达，但对cfos蛋白表达的影响与模型组比较，差异未见统计学意义。结论：活血潜阳方可以下调SHR心肌组织中原癌基因cmyc的表达，可能是改善高血压左心室肥厚的重要机制之一，是否通过对cfos表达的影响治疗左心室肥厚尚不能确定。 【关键词】 左心室肥厚; 自发性高血压大鼠; 活血潜阳方; 原癌基因蛋白质cfos; 原癌基因蛋白质cmyc 　　Objective: To explore the possible mechanism of Huoxue Qianyang Formula (HXQYF), a compound traditional Chinese herbal medicine, in reversing the left ventricular hypertrophy (LVH) of spontaneous hypertensive rats (SHRs) by analyzing the expressions of mRNAs and proteins of protooncogenes cfos and cmyc in left ventricular muscle.Methods: The experimental study was carried out in SHRs, the sex and agematched WistarKyoto (WKY) rats were served as normal control (n=5, normal saline 10 ml/kg daily). Twentyfive SHRs were randomly divided into five groups: untreated group (n=5, normal saline 10 ml/kg daily), highdose HXQYFtreated group (n=5, 0.84 g/ml HXQYF, 10 ml/kg daily), mediumdose HXQYFtreated group (n=5, 0.42 g/ml HXQYF, 10 ml/kg daily), lowdose HXQYFtreated group (n=5, 0.21 g/ml HXQYF, 10 ml/kg daily) and cilazapriltreated group (n=5, 1 mg/ml cilazapril, 10 ml/kg daily). The drugs were intragastrically administered once daily for 14 weeks. The expressions of mRNAs and proteins of protooncogenes cfos and cmyc in left ventricular muscle were detected separately by in situ hybridization histochemical method and immunohistochemical method.Results: Compared with the normal control group, the expressions of mRNAs and proteins of protooncogenes cfos and cmyc in left ventricular muscle were signific