氨基甾体类抗白血病化合物对K562白血病细胞系作用及其机理的研究.pdf

6 细胞内总[Ca2+]降低，但其对K562细胞的增殖和分化均无明显影响， 殖和诱导分化，那么氨基甾体H51817的作用可能还伴有其他机制的 参与，这有待进一步的深入研究。 关键词氨基甾体，白血病细胞，增殖，分化，钙离子 V ABSTRACT To the Objectiveexploreproliferation、and effectsand mechanismsofaminosteroidonhumam cing potencial chronic cell leukemiaK562line． myelogenous MethodsProliferationwasdetectedMTT and cell by assay by in both andsemisolid was counting liquid cultures．Differentiation measured and flow bymorphology,benzidine staining cytometry．The ofintracellularand were changes verifiedatomic extracellular[ca2+1by absorption andconfocal spectrometer,fluorescencespectrophotometer laster took arabinosideasthe scanningmicroscopy．Wecytosine positive contr01．Tosearchfor the betweentheeffectsofaminosteroid relationship onI(562cellline treatedK562cellswith and[ca2+】，we indomethacin， EGTAonK562ascontrols． BayK8644，and Results 1 Theeffectsof on aminosteroid anddifferentiation proliferation ofK562cells Proliferation：TheofK562cellswasinhibited growth except aminosteroidGaftertreatmentwith10一mol／Lof8differentaminoste． roidsfor5d．The K5