耐碳青霉烯类鲍曼不动杆菌耐药机制研究-中国感染与化疗杂志.pdfVIP

412 2007 12 20 7 6 Chin J Infect Chemother, Dec1 2007, Vol1 7, No1 6 # # * * 张雪云, 褚云卓 , 欧阳金鸣 , 陈佰义 : 20 , E ;( PFGE) ; ( IEF) PCR-(SDS) ( O P) 20 B ( 90%) ,4 0% IC 0. 125 mg/ L ;PFGE 5 , A1 ( 9 ), ICU ; 6. 7 , PCR OXA-23 , , Ñ, ;O P 31 ku , ; OXA-23 ;O P 3 1 ku ; O P : ; ; ; OXA-2 3 ; : R978 .17; R37 8 : A :100 9-7 708(2 00 7) 06-04 12-04 Study on the mechanism of carbapenem resi stance in Acinet obact er baumannii ZH A N G X ue-y un, CH U Yun-z h uo, O UYA N G J in-ming , CH EN Bai-y i. (D iv is ion o I n ec tious D ise ases , The First A il iate d H osp it al , Ch ina M edical Univ ers ity , Sheny ang 110001, Ch ina) Abstract: Obj ective T o investigatethe resistancepattern, homology and mechanism of carbapenem resistance inA cinetobacter baumannii. Methods Twenty carbapenem-resistant A . baumannii ( CRAB) isolates collected from 2 teaching hospitals of China edical University were evaluated for antibiotic sensitivity by E-test, homology by pulsed-field gel electrophoresis ( PFGE), and carbapenemases by isoelectric focusing electrophoresis (IEF), three dimensional tests, polymerase chain reaction ( PCR), cloning and sequencing methods. Outer membraneproteins were extracted by ultrasound physical method and analyzed by SDS- PAGE. Results CRAB were highly susceptibleto polymyxin B. Only 5 % of the isolates were resistant. The IC of moxifloxa- cin was lower than 0 .125 mg/L for 40 % of the CRAB isolates. According to PFGE, 20 CRAB isolates were typed as A, B, C, D and E, of which 9 belonged to typeA1, which were from ICU and Department of Respiratory edicine in the 2 teaching