响应面分析法优化桑叶叶绿素提取工艺-浙江大学学报.pdfVIP

浙江大学学报 （农业与生命科学版）38(6) ：725~731，2012 Journal of Zhejiang University (Agric. Life Sci.) /agr E-mail: zdxbnsb@ DOI: 10.3785/j.issn.1008-9209.2012.01.171 响应面分析法优化桑叶叶绿素提取工艺 陈绍瑗， 吕贞儿， 董峰丽， 毛碧增* （浙江大学农业与生物技术学院，浙江 杭州 310058 ） 摘要 为优化桑叶叶绿素提取工艺，根据单因素试验结果与响应面分析法中的Box-Behnken 中心组合设计原则选 取试验因素与水平，从中选取对叶绿素提取结果有明显影响的提取时间、提取温度、提取液料比3个因素进行优 化；并利用Design Expert 7.1.6分析软件对试验数据进行分析. 结果表明：桑叶叶绿素提取的最佳工艺参数为提取 时间5.25 h，提取温度56.5 °C，液料比103 ∶1；在此条件下，桑叶叶绿素质量分数为5.376 mg/g ，与预测值5.451 mg/g 接近. 说明根据Box-Behnken模型、采用响应面分析法得到的桑叶叶绿素提取优化工艺准确可靠. 关键词 桑叶； 叶绿素； 响应面分析 中图分类号 TS 201.1; S 888.2 文献标志码 A Optimization of chlorophyll extraction from mulberry leaves using response surface methodology. Journal of Zhejiang University (Agric. Life Sci.), 2012, 38(6): 725-731 CHEN Shao-yuan, LÜ Zhen-er, DONG Feng-li, MAO Bi-zeng* (College of Agriculture Biotechnology, Zhejiang University, Hangzhou 310058, China) Summary Chlorophyll plays an important role in our everyday life. It is used not only as an annexing agent in medicinal and calleidic products but also as an unartificial food coloring agent. Chlorophyll and its ramifications have antioxidant and anti-mutation properties. China has abundant mulberry resources. The mulberry leaf is an excellent Chinese herb from ancient times to the present day and contains many kinds of bioactive components, in which the chlorophyll content is an important physiological index. However, the optimization of chlorophyll extraction from mulberry leaf by response surface methodology (RSM) has not been reported so far. Response surface methodology (RSM) is an effectively statistical technique for optimi