餐厨垃圾中乳酸菌的筛选及其发酵条件的优化 screening of lactic acid bacteria in kitchen wastes and its fermentation conditions optimization.pdfVIP

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餐厨垃圾中乳酸菌的筛选及其发酵条件的优化 screening of lactic acid bacteria in kitchen wastes and its fermentation conditions optimization.pdf

餐厨垃圾中乳酸菌的筛选及其发酵条件的优化 screening of lactic acid bacteria in kitchen wastes and its fermentation conditions optimization

农产品工程 ·技术 TECHNIQUE 餐厨垃圾中乳酸菌的筛选及其发酵条件的优化 季 祥 乔春燕 蔡 禄 (内蒙古科技大学生物工程与技术研究所 , 内蒙古 包头 014010 ) 【摘要 】 从餐厨垃圾中分离出 3 种菌 , 通过形态观察 、 生理生化鉴定和乳酸定性试验筛选出一株具有溶钙 圈的乳酸菌 , 命名为 R , 分离纯化并保存 。 根据发酵液吸光度的测定值绘出该乳酸菌的生长曲线 , 选择对数期 的菌用于餐厨垃圾的乳酸发酵 。 采用单因素和正交试验对发酵条件进行优化 。 最后确定最佳发酵条件为 : 固液 比 1/9、 接种量 10%、 pH 值 6.0、 温度 43℃和发酵时间 60h 。 最终得出加入 R1 乳酸菌比不加 R1 乳酸菌的空白 对照的乳酸浓度高 15.5%。 【关键词 】 餐厨垃圾 ; 乳酸菌 ; 乳酸发酵 ; 条件优化 中图分类号 : S 646.1 文献标识码 : A 文章编号 : 2095-6495 (2014) 08-0061-05 Screening of Lactic Acid Bacteria in Kitchen Wastes and Its Fermentation Conditions Optimization Ji Xiang Qiao Chunyan Cai Lu ( , , Institute of Bioengineering and Technology Inner Mongolia University of Science and Technology Baotou City , Inner Mongolia 014010 ) Abstract : Three kinds of bacteria were separated out from kitchen wastes , a kind of lactic acid bacteria was screened out through morphology , physiological and biochemical identification , Lactic acid qualitative test , this lactic acid bacteria was named “R” , and then R was sumblimated and preserved. According to determination of absorbance values , lactic acid bacteria growth curve was drawn , choice of logarithm period was used for lactic acid fermentation of kitchen wastes. A single factor and orthogonal design was adopted to optimize fermentation conditions. Finally , optimal fer

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