餐厨垃圾中乳酸菌的筛选及其发酵条件的优化 screening of lactic acid bacteria in kitchen wastes and its fermentation conditions optimization.pdfVIP
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餐厨垃圾中乳酸菌的筛选及其发酵条件的优化 screening of lactic acid bacteria in kitchen wastes and its fermentation conditions optimization
农产品工程 ·技术 TECHNIQUE
餐厨垃圾中乳酸菌的筛选及其发酵条件的优化
季 祥 乔春燕 蔡 禄
(内蒙古科技大学生物工程与技术研究所 , 内蒙古 包头 014010 )
【摘要 】 从餐厨垃圾中分离出 3 种菌 , 通过形态观察 、 生理生化鉴定和乳酸定性试验筛选出一株具有溶钙
圈的乳酸菌 , 命名为 R , 分离纯化并保存 。 根据发酵液吸光度的测定值绘出该乳酸菌的生长曲线 , 选择对数期
的菌用于餐厨垃圾的乳酸发酵 。 采用单因素和正交试验对发酵条件进行优化 。 最后确定最佳发酵条件为 : 固液
比 1/9、 接种量 10%、 pH 值 6.0、 温度 43℃和发酵时间 60h 。 最终得出加入 R1 乳酸菌比不加 R1 乳酸菌的空白
对照的乳酸浓度高 15.5%。
【关键词 】 餐厨垃圾 ; 乳酸菌 ; 乳酸发酵 ; 条件优化
中图分类号 : S 646.1 文献标识码 : A 文章编号 : 2095-6495 (2014) 08-0061-05
Screening of Lactic Acid Bacteria in Kitchen Wastes and
Its Fermentation Conditions Optimization
Ji Xiang Qiao Chunyan Cai Lu
( , ,
Institute of Bioengineering and Technology Inner Mongolia University of Science and Technology
Baotou City , Inner Mongolia 014010 )
Abstract : Three kinds of bacteria were separated out from kitchen wastes , a kind of lactic acid bacteria was
screened out through morphology , physiological and biochemical identification , Lactic acid qualitative test , this lactic
acid bacteria was named “R” , and then R was sumblimated and preserved. According to determination of absorbance
values , lactic acid bacteria growth curve was drawn , choice of logarithm period was used for lactic acid fermentation of
kitchen wastes. A single factor and orthogonal design was adopted to optimize fermentation conditions. Finally , optimal
fer
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