人参与胡萝卜愈伤组织共培养的研究.PDFVIP

※基础研究 食品科学 2008, Vol. 29, No. 01 55 人参与胡萝卜愈伤组织共培养的研究 杨 晶，张美萍，王 义* (吉林农业大学生命科学学院，吉林 长春 130118) 摘 要：本研究采用正交试验设计，对人参(Panax ginseng)和胡萝卜(Daucus carota)两种植物组织共培养的培养基 和培养条件进行了优化筛选，同时比较了人参愈伤组织与共培养物的皂苷含量。结果表明，人参与胡萝卜共培养 所获得的愈伤组织在23℃，1000lx的条件下生长良好，最佳基质MS+2,4-D 3mg/L+IBA 2mg/L+KT 0.5mg/L，在培 养25d时皂苷含量达到最高，浓度达到 1.6mg/L。共培养物的皂苷含量比人参愈伤组织皂苷含量高出6.25%，为人 参与胡萝卜原生质体融合、获得营养型蔬菜奠定了基础。 关键词：人参；胡萝卜；共培养；人参皂苷 Study on Callus Co-culture of Panax ginseng and Daucus carota YANG Jing，ZHAGN Mei-ping，WANG Yi* (College of Life Science, Jilin Agricultural University, Changchun 130118, China) Abstract ：With orthogonal test design, to optimize bolting of medium and culture conditions with co-culture of Panax ginseng and Daucus carota, the saponin contents of Panax ginseng and co-culture were simultaneously compared. The results showed Panax ginseng and Daucus carota grow well on the condition of 23 ℃ and 1000 lx with the that the callus of the co-culture of optimal medium MS+2,4-D 3 mg/L+IBA 2 mg/L+KT 0.5 mg/L. After 25 d culture, the saponin content of co-culture is 6.25% Panax ginseng callus. It settles foundation for protoplast fusion of Panax ginseng and Daucus carota as to higher than that of obtain trophic vegetables. Panax ginseng；Daucus carota；co-culture；Panax saponin Key words： 中图分类号：Q943.1 文献标识码：A 文章编号：1002-6630(2008)01-0055-05 人参是我国名贵中药，堪称当今天然补益药的代 养型蔬菜，为进一步研究人参与胡萝卜原生质体融合 表。享有中药之王的美誉，久为世人瞩目，已有两千 奠定基础。 多年的应用历史。它具有增强免疫力、抗疲劳、保护