原创力文档酸解玉米秸秆糖化菌株绿色木霉Trichodermaviride NUA-051的选育及摇瓶发酵条件 Selection of Trichodermaviride NUA-051 for saccharification of acidolysismaize straws and its fermentation technologies in shake-flask culture.pdfVIP
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酸解玉米秸秆糖化菌株绿色木霉Trichodermaviride NUA-051的选育及摇瓶发酵条件 Selection of Trichodermaviride NUA-051 for saccharification of acidolysismaize straws and its fermentation technologies in shake-flask culture
一墨础理论
酸解玉米秸秆糖化菌株绿色木霉
TrichodermavirideNUA一05
1的
选育及摇瓶发酵条件
李亚蕾1，杨波2，罗瑞明1
(1．宁夏大学农学院，银川750021；2．宁夏大学生命科学学院，银川750021)
摘要：取一定量的玉米秸秆粉与水按1：4～8混匀，加入适量3rnol／L硫酸使混合物的pH值为2～3，常温
25℃处理48h，用浓氨水中和至pH=5．0。以正交实验优选发酵菌株、培养基组成及培养条件。实验表
20
(NH4)2S04g／L，酸解秸秆含量1509／L，温度35℃，
明：采用绿色木霉(Trichodermaviride)3．2774，
酵糖化率40．7％～45．3％．具有遗传稳定性，为秸秆发酵生产单细胞蛋白奠定了基础。
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