为何不用淀粉酶鉴定PH对酶活性的影响.docVIP

精品论文 参考文献 为何不用淀粉酶鉴定PH对酶活性的影响 【摘 要】《影响酶活性条件》的探究实验是高中生物人教版必修①中学生必修的探究实验，课本用括号注明：建议用淀粉酶探究温度对酶活性的影响，用过氧化氢酶探究PH对酶活性的影响。我想为什么不也用淀粉酶来探究PH对酶活性的影响呢？两个探究实验同时用一种溶液—H2O2溶液，准备起来简单方便，且经济。如果配置过氧化氢溶液的同时，再配置肝脏研磨液，不但增加了工作量，而且浪费。经过试验，我发现用淀粉酶探究PH对酶活性的影响试验过程中，出现了出人意料的实验现象，尽管能够解释PH对酶的活性有影响，但因为学生缺乏相应的化学知识，不容易解释明白。本人在试验的基础上找出了课本注明的原因，希望对同行老师们少走一点弯路。 【关键词】淀粉；淀粉酶；NaOH；HCI；碘—碘化钾溶液 Why does need not starch Mao authenticate PH to influence Mao activity Li Xing-zhen 【Abstract】《Influence Mao activity condition 》of investigation experiment is senior high school living creature the person teach a version a required ① high school student required of investigation experiment, lesson origin note with the brackets:The suggestion use starch Mao an investigation temperature to the Mao activity of influence, once used to oxidize hydrogen Mao investigation PH to the Mao activity of influence.Do I think why to dont also influence Mao activity with the starch Mao investigation PH?Two investigation experiment use a kind of aqua-H2 O2 aqua in the meantime, prepare to be simple convenience, and economy.If allocation lead oxidize hydrogen aqua of in the meantime, again allocation the liver grind a liquid, not only increment workload, and waste.Has been experiment, I detection use starch Mao investigation PH to the Mao activity of influence experiment process in, appeared by surprise of experiment phenomenon, though can explanation PH to Mao of activity have influence, because of the student lack correspond of chemistry knowledge, not easy explanation understand.Oneself on the foundation for experiment found out lesson origin note of reason, hope to go together the teachers little walk 1:00 curved road. 【Key words】Starch;Starch Mao;NaOH;HCI;The iodine-iodine turn potassium aqua 《影响酶活性条件》的探究实验是高中生物人民教育出版社出版的必修①《分子与细胞》中学生必修的探究实验，在课本第84页用括号注明：建议用淀粉酶探究温度对酶活性的影响，用过氧化氢酶探究PH对酶活性的影响。既然探究影响酶活性的条件，不管用哪一种酶作为实验材料，只要实验结果能说明问题就可以了，何必要建议呢？更何况如果按照课本建议去做，实验准备工作，实验完毕后的清理工作费时费力，因为选用过氧化氢酶作为实验材料，在购买新鲜的动物肝脏后，需要研磨、分装，特别是实验后的清理工作很是麻烦（因