烟草野火病病原菌对农用链霉素的抗药性测定.docVIP

烟草野火病病原菌对农用链霉素的抗药性测定 ?328? ChineseAgriculturalScienceBulletinVo1.23No.122007December 烟草野火病病原茵对农用链霉素的抗药性测定 李梅云 (云南省烟草科学研究所,玉溪653100) 摘要:为合理防治烟草野火病,减少抗药性产生,减少农药残留.从云南省8个地州分离烟草野火病茵 茵株43个,并就分离茵株对农用链霉素的抗药性进行了测定,结果表明,供试43个茵株中高抗茵株8 个,中抗茵株比例较高17个,低抗茵株18个.不同来源的烟草野火病原茵株的抗药性存在差异,文山, 昆明的茵株抗药性最高,红河茵株抗药性最低,抗性倍数最高达2000多倍,其余地州抗药性居中.不同 地州分离菌株高,中,低抗茵株的比例存在明显差另l】.分离自文山邱北县双龙营镇的25#茵株抗药性最 强.抗性倍数达45907.75,来自文山砚山县阿猛镇租白村的34#茵株抗药性最低.43株烟草野火病茵茵 株对农用链霉素的EC50值平均为4.65,范围在0.004~183.631.高,中,低抗茵株的平均抗性倍数分别为 6124.69,49.72,6.40. 关键词:烟草野火病;农用链霉素;抗药性 中图分类号:$572文献标识码:A DeterminationofPseudomonassy~ngaepv.tabaciResistance toAgriculturalStreptomycin LiMeiyun (TobaccoScienceResearchInstitute,YunnanYuxi653100) Abstract:Tocontroltobaccowildfirediseaserationally,reduceresistanceandreducepesticidepollution, 43strainsofPseudomonassyringaepv.tabaciwereisolatedfrom8regionofYunnanprovinceandalli8o— latesweredeterminedtheirresistancetoagriculturalstreptomycin.Theresultsindicatedthatofalltestediso— lates,therewere8highresistance,17middleresistanceand18lowresistanceisolatesrespectively.I’}leis1).- latesfungicideresistancefromdifferentregionwasdifferentobviously,thehighestresistantisolatecomefrom WenshanandKunmingregion.Hongheisolateshadthelowestresistanceamongalltestedisolates.I1leevi- dentdisparityofhigh—resistance.mid—resistanceandlow—resistanceisolatesratiowasexistatdifferentre— gion.25#fromWenshanQiubeiwasthehighestagriculturalstreptomycinresistantisolate,itsresistance multiplearrivedto45908comparedtothelowestresistanceisolate34#,whichfromYanshanofWenshan. I1leEC50of43Pseudomonassyringaepv.tabaciisolatesagainstagriculturalstreptomycinwas4.65inaver- age.wasintherangeofO.004-183.631.,I1leaverageresistancemultipleofhigh—resistance,mid—resistance andlow-resistanceisolateswas6124.69,49.72,6.40respectively. Keywords:tobaccowildfirediseases,agriculturalstreptomycin,fungicideresistance 烟草野火病是由烟草假单胞杆菌(Pseudomonas syringaepv.tabaci)~[起的细菌性病害,是一种世界普 遍发生的爆发性,毁灭性病害m,Wolf和Foser首次报 道报道在美国北卡罗来纳州【’].该病害于2O世纪4O 年代末期在中国云南烟区零星发生,以后随着